Tagliavini F, Giaccone G, Linoli G, Frangione B, Bugiani O
Istituto Neurologico Carlo Besta, Milano, Italy.
Prog Clin Biol Res. 1989;317:1001-5.
Using anti-SP28 (a polyclonal antibody to a 28 residue synthetic peptide homologous to the NH2-terminal region of Alzheimer amyloid beta-protein) to investigate the cerebral cortex of 8 Alzheimer and 6 Down patients and 13 nondemented individuals, we found that, besides senile plaques and congophilic vessels, extracellular deposits unrelated to degenerating neurites, tangle-bearing neurons or congophilic vessels were labelled. These deposits were observed in all Alzheimer and 4 Down patients, and in 5 nondemented individuals. The material accumulated in these deposits did not react with Congo red, thioflavine S or, on some occasions, silver salts and therefore might have been constituted by beta-protein and/or beta-protein precursors lacking the conformation of amyloid fibrils.
使用抗SP28（一种针对与阿尔茨海默病淀粉样β蛋白NH2末端区域同源的28个残基合成肽的多克隆抗体）研究8例阿尔茨海默病患者、6例唐氏综合征患者和13名非痴呆个体的大脑皮质，我们发现，除了老年斑和嗜刚果红血管外，与神经突退变、含缠结神经元或嗜刚果红血管无关的细胞外沉积物也被标记。在所有阿尔茨海默病患者和4例唐氏综合征患者以及5名非痴呆个体中均观察到这些沉积物。这些沉积物中积累的物质不与刚果红、硫黄素S反应，在某些情况下也不与银盐反应，因此可能由缺乏淀粉样纤维构象的β蛋白和/或β蛋白前体组成。
