Tagliavini F, Giaccone G, Frangione B, Bugiani O
Division of Neuropathology, Istituto Neurologico Carlo Besta, Milan, Italy.
Neurosci Lett. 1988 Nov 11;93(2-3):191-6. doi: 10.1016/0304-3940(88)90080-8.
A polyclonal antibody to a 28 residue synthetic peptide, homologous to the NH2 terminal region of amyloid beta-protein, was employed in a study of the frontal and temporal cortex of 8 Alzheimer patients and 13 non-demented individuals aimed to define the relationship of immunolabelled to argyrophilic, congophilic and thioflavine S-positive cortical lesions. In Alzheimer patients, this antiserum labelled not only senile plaques and congophilic angiopathy, but also cortical deposits that were neither argyrophilic, congophilic nor thioflavine S-positive and were unrelated to degenerating neurites, tangle-bearing neurons or congophilic angiopathy. Similar lesions were observed in 4 of 13 non-demented individuals, in the absence of tangles, plaques or congophilic angiopathy, and in one in association with plaques. Such deposits might have been due to amyloid precursors still lacking the beta-pleated sheet molecular conformation responsible for amyloid tinctorial and optical properties.
一种针对与β-淀粉样蛋白NH2末端区域同源的28个残基合成肽的多克隆抗体，被用于一项对8名阿尔茨海默病患者和13名非痴呆个体的额叶和颞叶皮质的研究，旨在确定免疫标记与嗜银性、嗜刚果红性和硫黄素S阳性皮质病变之间的关系。在阿尔茨海默病患者中，这种抗血清不仅标记了老年斑和嗜刚果红性血管病，还标记了既非嗜银性、也非嗜刚果红性、亦非硫黄素S阳性且与神经突退变、含缠结神经元或嗜刚果红性血管病无关的皮质沉积物。在13名非痴呆个体中的4名中观察到了类似病变，这些个体不存在缠结、斑块或嗜刚果红性血管病，其中1名个体的病变与斑块有关。这种沉积物可能是由于淀粉样前体仍缺乏负责淀粉样染色和光学特性的β-折叠片层分子构象。
