Quantitative Crop Genetics, Max Planck Institute for Plant Breeding Research Cologne, Germany ; Crop Genetics and Biotechnology Unit, Institute of Crop Science and Resource Conservation, University of Bonn Bonn, Germany.
Saskatoon Research Centre, Agriculture and Agri-Food Canada Saskatoon, SK, Canada.
Front Plant Sci. 2014 Sep 30;5:485. doi: 10.3389/fpls.2014.00485. eCollection 2014.
Knowing the genetic basis of the plant ionome is essential for understanding the control of nutrient transport and accumulation. The aim of this research was to (i) study mineral nutrient concentrations in a large and diverse set of Brassica napus, (ii) describe the relationships between the shoot ionome and seedling development, and (iii) identify genetic regions associated with variation of the shoot ionome. The plant material under study was a germplasm set consisting of 509 inbred lines that was genotyped by a 6K single nucleotide polymorphism (SNP) array and phenotyped by analyzing the concentrations of eleven mineral nutrients in the shoots of 30 days old seedlings. Among mineral concentrations, positive correlations were found, whereas mineral concentrations were mainly negatively correlated with seedling development traits from earlier studies. In a genome-wide association mapping approach, altogether 29 significantly associated loci were identified across seven traits after correcting for multiple testing. The associations included a locus with effects on the concentrations of Cu, Mn, and Zn on chromosome C3, and a genetic region with multiple associations for Na concentration on chromosome A9. This region was situated within an association hotspot close to SOS1, a key gene for Na tolerance in plants.
了解植物矿质元素组的遗传基础对于理解养分运输和积累的调控至关重要。本研究的目的是：（i）研究大量不同甘蓝型油菜的矿质养分浓度，（ii）描述地上部矿质元素组与幼苗发育之间的关系，以及（iii）鉴定与地上部矿质元素组变异相关的遗传区域。所研究的植物材料是一个种质资源集，由 509 个自交系组成，这些自交系通过 6K 单核苷酸多态性（SNP）阵列进行基因型分析，并通过分析 30 天大幼苗地上部 11 种矿质养分的浓度进行表型分析。在矿质浓度中，发现了正相关关系，而矿质浓度主要与早期研究中幼苗发育性状呈负相关。在全基因组关联作图方法中，在对多次检验进行校正后，总共在七个性状中共鉴定出 29 个显著关联的位点。这些关联包括一个对 C3 染色体上 Cu、Mn 和 Zn 浓度有影响的位点，以及一个对 A9 染色体上 Na 浓度有多个关联的遗传区域。该区域位于 SOS1 附近的一个关联热点内，SOS1 是植物耐钠的关键基因。
