果蝇中硫酸乙酰肝素功能研究的遗传学方法

Genetic approaches in the study of heparan sulfate functions in Drosophila.

作者信息

Takemura Masahiko, Nakato Hiroshi

机构信息

Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN, 55455, USA.

出版信息

Methods Mol Biol. 2015;1229:497-505. doi: 10.1007/978-1-4939-1714-3_38.

DOI:10.1007/978-1-4939-1714-3_38

PMID:25325975

Abstract

Several classes of heparan sulfate proteoglycan (HSPG) core proteins and all HS biosynthetic/modifying enzymes are evolutionarily conserved from human to Drosophila melanogaster. This genetically tractable model offers highly sophisticated techniques to manipulate gene function in a spatially and temporally controlled manner. Thus, Drosophila has been a powerful system to explore the functions of HSPGs in vivo. In this chapter, we will introduce two genetic techniques available in Drosophila: TARGET (temporal and regional gene expression targeting) and MARCM (mosaic analysis with a repressible cell marker).

摘要

几类硫酸乙酰肝素蛋白聚糖（HSPG）核心蛋白以及所有硫酸乙酰肝素生物合成/修饰酶在从人类到黑腹果蝇的进化过程中都是保守的。这种具有遗传易处理性的模型提供了高度精密的技术，能够以空间和时间可控的方式操纵基因功能。因此，果蝇一直是在体内探索硫酸乙酰肝素蛋白聚糖功能的强大系统。在本章中，我们将介绍果蝇中可用的两种遗传技术：TARGET（时空基因表达靶向）和MARCM（带有可抑制细胞标记的镶嵌分析）。