Takemura Masahiko, Nakato Hiroshi
Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN, 55455, USA.
Methods Mol Biol. 2015;1229:497-505. doi: 10.1007/978-1-4939-1714-3_38.
Several classes of heparan sulfate proteoglycan (HSPG) core proteins and all HS biosynthetic/modifying enzymes are evolutionarily conserved from human to Drosophila melanogaster. This genetically tractable model offers highly sophisticated techniques to manipulate gene function in a spatially and temporally controlled manner. Thus, Drosophila has been a powerful system to explore the functions of HSPGs in vivo. In this chapter, we will introduce two genetic techniques available in Drosophila: TARGET (temporal and regional gene expression targeting) and MARCM (mosaic analysis with a repressible cell marker).
几类硫酸乙酰肝素蛋白聚糖（HSPG）核心蛋白以及所有硫酸乙酰肝素生物合成/修饰酶在从人类到黑腹果蝇的进化过程中都是保守的。这种具有遗传易处理性的模型提供了高度精密的技术，能够以空间和时间可控的方式操纵基因功能。因此，果蝇一直是在体内探索硫酸乙酰肝素蛋白聚糖功能的强大系统。在本章中，我们将介绍果蝇中可用的两种遗传技术：TARGET（时空基因表达靶向）和MARCM（带有可抑制细胞标记的镶嵌分析）。
