用于果蝇中蛋白聚糖功能的分子遗传技术。
Molecular Genetic Techniques for the Proteoglycan Functions in Drosophila.
机构信息
Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN, USA.
出版信息
Methods Mol Biol. 2022;2303:405-414. doi: 10.1007/978-1-0716-1398-6_32.
Abstract
Several classes of heparan sulfate proteoglycan (HSPG) core proteins and all HS biosynthetic/modifying enzymes are evolutionarily conserved from human to Drosophila melanogaster. This genetically tractable model offers highly sophisticated techniques to manipulate gene function in a spatially and temporally controlled manner. Thus, Drosophila genetics has been a powerful system to explore functions of HSPGs in vivo. In this chapter, we will introduce three genetic techniques available in Drosophila: TARGET (temporal and regional gene expression targeting), MARCM (mosaic analysis with a repressible cell marker), and FLP-Out.
摘要
几种硫酸乙酰肝素蛋白聚糖 (HSPG) 核心蛋白和所有 HS 生物合成/修饰酶在人类到黑腹果蝇的进化过程中都是保守的。这种遗传上可操作的模型提供了高度复杂的技术，可以以空间和时间控制的方式操纵基因功能。因此，果蝇遗传学一直是探索 HSPG 体内功能的强大系统。在本章中，我们将介绍果蝇中可用的三种遗传技术：TARGET（时间和区域基因表达靶向）、MARCM（可抑制细胞标记的马赛克分析）和 FLP-Out。
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