硫酸乙酰肝素调节Slit3诱导的内皮细胞迁移。

Heparan sulfate modulates Slit3-induced endothelial cell migration.

作者信息

Qiu Hong, Xiao Wenyuan, Yue Jingwen, Wang Lianchun

机构信息

Department of Biochemistry and Molecular Biology, Complex Carbohydrate Research Center, University of Georgia, 315 Riverbend Road, Athens, GA, 30602-4712, USA.

出版信息

Methods Mol Biol. 2015;1229:549-55. doi: 10.1007/978-1-4939-1714-3_43.

DOI:10.1007/978-1-4939-1714-3_43

PMID:25325980

Abstract

Heparan sulfate is a long, linear polysaccharide with sulfation modifications and belongs to the glycosaminoglycan family. Our recent studies elucidated that the axon guidance molecule Slit3 is a new heparan sulfate-binding protein and a novel angiogenic factor by interacting with its cognate receptor Robo4, which is specifically expressed in endothelial cells. Here we describe using heparan sulfate-deficient mouse endothelial cells to determine the co-reception function of heparan sulfate in Slit3-induced endothelial cell migration in a Boyden chamber trans-well migration assay.

摘要

硫酸乙酰肝素是一种具有硫酸化修饰的长链线性多糖，属于糖胺聚糖家族。我们最近的研究表明，轴突导向分子Slit3是一种新的硫酸乙酰肝素结合蛋白，也是一种新型血管生成因子，它通过与其在内皮细胞中特异性表达的同源受体Robo4相互作用发挥作用。在此，我们描述了在博伊登室跨膜迁移试验中，使用硫酸乙酰肝素缺陷型小鼠内皮细胞来确定硫酸乙酰肝素在Slit3诱导的内皮细胞迁移中的共受体功能。