Kiloniella spongiae sp. nov., isolated from a marine sponge and emended description of the genus Kiloniella Wiese et al. 2009 and Kiloniella laminariae.

A Gram-reaction-negative, aerobic, rod-shaped and non-motile marine bacterium, designated MEBiC09566(T) was isolated from a sponge collected at Uljin County in the coastal area of the East Sea (36° 55' N, 129° 25' E), Korea. The 16S rRNA gene sequence analysis revealed that strain MEBiC09566(T) showed the highest similarity with the Kiloniella laminariae LD81(T) (96.7%). Growth was observed at 11-31 °C (optimum 25 °C), at pH 6.0-8.5 (optimum pH 7.0) and with 0-6% (optimum 2.5%) NaCl. The predominant cellular fatty acids were summed feature 8 (comprised of C18:1ω7c/C18:1ω6c) and summed feature 3 (comprised of C16:1ω7c and/or C16:1ω6c). The DNA G+C content is 44.6 mol%. The major respiratory quinone is Q-9. Phosphatidylethanolamine, phosphatidylglycerol, an unidentified lipid, two unidentified aminophospholipids and one unidentified aminolipid were detected as major polar lipids. On the basis of this polyphasic taxonomic data, it is concluded that strain MEBiC09566(T) should be classified as representing a novel species in the genus Kiloniella and the name proposed is Kiloniella spongiae sp. nov. The type strain is MEBiC09566(T) ( = KCCM 43040(T) = JCM 19930(T)). Emended descriptions of the genus Kiloniella Wiese et al. 2009 and Kiloniella laminariae are also given.