2Fujian Province University Technology and Engineering Center for Marine Biomedical Resource Utilization, Xiamen Medical College, Xiamen, 361023, PR China.
1Xiamen Key Laboratory of Marine Medicinal Natural Products Resources, Xiamen Medical College, 361023, PR China.
Int J Syst Evol Microbiol. 2019 Jul;69(7):2043-2048. doi: 10.1099/ijsem.0.003423.
A taxonomic study was carried out on strain HN-Y73, which was isolated from sponge collected from Yangpu Bay, Hainan, PR China. Strain HN-Y73 was Gram-stain-negative, non-motile, ovoid- to rod-shaped, yellow-pigmented and could grow at 10-42 °C (optimum, 28 °C), at pH 5.0-9.0 (pH 8.0) and in 0.5-16 % (w/v) NaCl (3-4 %). This isolate was positive for oxidase, catalase and the hydrolysis of aesculin and xylan, but negative for the hydrolysis of casein and gelatin. Strain HN-Y73 shared the highest 16S rRNA gene sequence similarity with Altererythrobacter endophyticus BR75 (96.9 %), followed by Altererythrobacter estronivorus MH-B5 (96.0 %), Altererythrobacter indicus MSSRF26 (95.9 %) and Altererythrobacter mangrovi C9-11 (95.7 %). The phylogenetic tree showed that strain HN-Y73 forms a clade with A. endophyticus BR75, A. indicus MSSRF26 and A. estronivorus MH-B5 within the genus Altererythrobacter. The dominant fatty acids were summed feature 8 (C18 : 1ω7c/ω6c) and C19 : 0cyclo ω8c. The major polar lipids comprised phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine, sphingoglycolipid phosphatidyldimethylethanolamine, phosphatidylmonomethylethanolamine, one unidentified glycolipid, two unidentified phospholipids and four unidentified lipids. The respiratory lipoquinone was identified as Q-10. The G+C content of the genomic DNA was 57.6 mol%. Based on the phenotypic and phylogenetic data, strain HN-Y73 represents a novel species of the genus Altererythrobacter, for which the name Altererythrobacter spongiae sp. nov. is proposed. The type strain is HN-Y73 (=MCCC 1K03324=LMG 30461).
对一株分离自中国海南杨浦湾海绵的菌株 HN-Y73 进行了分类学研究。菌株 HN-Y73 革兰氏阴性，非运动性，呈卵形到杆状，呈黄色色素，可在 10-42°C（最佳温度为 28°C）、pH5.0-9.0（pH8.0）和 0.5-16%（w/v）NaCl（3-4%）下生长。该分离株氧化酶、过氧化氢酶和 aesculin 和木聚糖水解阳性，但酪蛋白和明胶水解阴性。菌株 HN-Y73 的 16S rRNA 基因序列与内共生的 Altererythrobacter endophyticus BR75 的相似度最高（96.9%），其次是 Altererythrobacter estronivorus MH-B5（96.0%）、 Altererythrobacter indicus MSSRF26（95.9%）和 Altererythrobacter mangrovi C9-11（95.7%）。系统发育树显示，菌株 HN-Y73与内共生的 Altererythrobacter endophyticus BR75、Altererythrobacter indicus MSSRF26 和 Altererythrobacter estronivorus MH-B5 一起形成了一个属内的分支。优势脂肪酸为 sum 特征 8（C18:1ω7c/ω6c）和 C19:0cyclo ω8c。主要极性脂包括磷脂酰乙醇胺、磷脂酰甘油、双磷脂酰甘油、磷脂酰胆碱、神经酰胺糖脂磷脂酰二甲乙醇胺、磷脂酰单甲乙醇胺、一种未鉴定的糖脂、两种未鉴定的磷脂和四种未鉴定的脂类。呼吸类脂醌鉴定为 Q-10。基因组 DNA 的 G+C 含量为 57.6mol%。基于表型和系统发育数据，菌株 HN-Y73 代表了 Altererythrobacter 属的一个新种，建议将其命名为 Altererythrobacter spongiae sp. nov.。模式菌株为 HN-Y73（=MCCC 1K03324=LMG 30461）。
