Heilig M, Bäuml D, Fürll M
Mandy Heilig, Dürrenberger Straße 12, 04177 Leipzig, E-Mail:
Tierarztl Prax Ausg G Grosstiere Nutztiere. 2014;42(5):261-70.
Frequent occurrence of parturient paresis (milk fever [MF]) and a partly unsatisfactory treatment success raises the question as to whether in addition to the known causes, other factors influence the incidence and success of MF treatment. Trace elements, including selenium (Se), are involved in bone metabolism, however, there is little knowledge regarding the influence of Se on MF development. The aim of this study was to analyse the concentrations of Se and the statistical relationships to parameters associated with Se influence in downer cows.
A total of 195 Simmental cows, downer cows and clinically healthy control animals were divided into five groups: a) control group (CG, n = 21), b) all cows with MF (n = 174), c) MF cows without additional diseases (n = 145), d) cows with MF and mastitis (n = 10) and e) cows with MF and retained placenta or endometritis (n = 19). Se, calcium (Ca), inorganic phosphate (Pi), tumour necrosis factor α (TNFα), haptoglobin (Hp), antioxidants (Trolox Equivalent Antioxidative Capacity: TEAC) and further metabolic parameters were analysed in the blood serum.
The serum concentrations of Se, Ca, Pi and TEAC were lower in groups b) to e), whereas Hp was higher than in the CG (p ≤ 0.05). Se was positively correlated with Pi in the CG, in groups b) and c) with Ca, Pi, K and Mg and in group c) with Hp (p ≤ 0.05). Both Ca and Pi were significantly lower in group c) compared to group d) (p ≤ 0.05). TNFα was increased in groups b) and c) compared to group a) and correlated with Se in group e) (p ≤ 0.05). Alkaline phosphatase activity in groups b) and e) was lower than in the CG and correlated with Se in the CG and group b) (p ≤ 0.05).
These results, in agreement with the literature data, support the hypothesis that Se could be directly involved in bone metabolism and therefore in the pathogenesis of MF. Se acts via cytokines on Ca mobilization from bone. The concentrations of Hp and TEAC support this interpretation. Therefore, control of the Se status and Se supplementation of cows should be included in the prevention and advanced therapy of MF.
分娩瘫痪（乳热症[MF]）频繁发生且治疗成功率部分不尽人意，这引发了一个问题，即除了已知原因外，是否还有其他因素影响MF的发病率和治疗成功率。包括硒（Se）在内的微量元素参与骨骼代谢，然而，关于Se对MF发展的影响知之甚少。本研究的目的是分析Se的浓度以及与产犊母牛中Se影响相关参数的统计关系。
总共195头西门塔尔牛、产犊母牛和临床健康对照动物被分为五组：a）对照组（CG，n = 21），b）所有患有MF的母牛（n = 174），c）无其他疾病的MF母牛（n = 145），d）患有MF和乳腺炎的母牛（n = 10），e）患有MF和胎盘滞留或子宫内膜炎的母牛（n = 19）。分析血清中的Se、钙（Ca）、无机磷（Pi）、肿瘤坏死因子α（TNFα）、触珠蛋白（Hp）、抗氧化剂（Trolox等效抗氧化能力：TEAC）和其他代谢参数。
b）至e）组血清中Se、Ca、Pi和TEAC的浓度较低，而Hp高于CG组（p≤0.05）。在CG组中，Se与Pi呈正相关，在b）组和c）组中与Ca、Pi、K和Mg呈正相关，在c）组中与Hp呈正相关（p≤0.05）。与d）组相比，c）组的Ca和Pi均显著降低（p≤0.05）。与a）组相比，b）组和c）组的TNFα升高，且在e）组中与Se相关（p≤0.05）。b）组和e）组的碱性磷酸酶活性低于CG组，且在CG组和b）组中与Se相关（p≤0.05）。
这些结果与文献数据一致，支持以下假设，即Se可能直接参与骨骼代谢，因此参与MF的发病机制。Se通过细胞因子作用于骨钙动员。Hp和TEAC的浓度支持这一解释。因此，在MF的预防和进阶治疗中应包括对母牛Se状态的控制和Se补充。
