a Department of College of Animal Science and Veterinary Medicine , Heilongjiang BaYi Agriculture University , Daqing 163319 , China.
Vet Q. 2014;34(3):159-63. doi: 10.1080/01652176.2014.924642. Epub 2014 Jun 13.
Milk fever (MF) is a common calcium metabolism disorder in perinatal cows. Currently, information regarding the detailed metabolism in cows suffering from MF is scant.
The purpose was to study the metabolic profiling of serum samples from cows with MF in comparison to control cows, and thereby exploring other underlying pathological mechanisms of this disease.
In the current study, we compared the serum metabolomic profile of dairy cows with MF (n = 8) to that of healthy dairy cows (n = 24) using a 500-MHz digital (1)H-nuclear magnetic resonance ((1)H-NMR) spectrometer. Based on their clinical presentation and serum calcium concentration, cows were assigned either to the control group (no MF symptoms and serum calcium concentration >2.5 mmol/L) or to the MF group (MF symptoms and serum calcium concentration <1.4 mmol/L). For statistical analysis, a one-way analysis of variance was performed.
We identified differences regarding nine metabolites between the two groups, among which glucose, alanine, glycerol, phosphocreatine, and gamma-aminobutyrate decreased, and β-hydroxybutyrate, acetone, pyruvate, and lysine increased in cows with MF. Most of these were carbohydrates and amino acids involved in various energy metabolism pathways.
The different metabolites in cows with MF reflected the pathological features of negative energy balance and fat mobilization, suggesting that MF is associated with altered energy metabolism.
The (1)H-NMR spectroscopy can be used to understand the pathogenesis of MF and identify biomarkers of the disease.
奶牛产后瘫痪（MF）是围产期奶牛常见的钙代谢紊乱疾病。目前，有关 MF 奶牛详细代谢情况的信息很少。
旨在研究 MF 奶牛与对照奶牛的血清代谢组学特征，从而探讨该疾病的其他潜在病理机制。
在本研究中，我们使用 500-MHz 数字（1）H-核磁共振（1H-NMR）谱仪比较了 8 头患有 MF 的奶牛和 24 头健康奶牛的血清代谢组学特征。根据其临床表现和血清钙浓度，将奶牛分为对照组（无 MF 症状且血清钙浓度＞2.5mmol/L）或 MF 组（MF 症状且血清钙浓度＜1.4mmol/L）。为了进行统计分析，我们进行了单向方差分析。
我们在两组之间鉴定出 9 种代谢物存在差异，其中葡萄糖、丙氨酸、甘油、磷酸肌酸和γ-氨基丁酸降低，β-羟丁酸、丙酮、丙酮酸和赖氨酸增加。这些代谢物大多是参与各种能量代谢途径的碳水化合物和氨基酸。
MF 奶牛的不同代谢物反映了负能量平衡和脂肪动员的病理特征，表明 MF 与能量代谢改变有关。
1H-NMR 光谱可用于了解 MF 的发病机制并鉴定疾病的生物标志物。
