采用不同溶剂从石榴叶中提取和定量测定酚酸和类黄酮。
Extraction and quantification of phenolic acids and flavonols from Eugenia pyriformis using different solvents.
机构信息
Programa de Pós-Graduação em Tecnologia de Alimentos (PPGTA), Universidade Tecnológica Federal do Paraná (UTFPR), Campus Campo Mourão, PR Brasil.
Programa de Pós-Graduação de Tecnologia de Processos Químicos e Bioquímicos (PPGTP), Universidade Tecnológica Federal do Paraná (UTFPR), Campus Pato Branco, PR Brasil.
出版信息
J Food Sci Technol. 2014 Oct;51(10):2862-6. doi: 10.1007/s13197-012-0759-z. Epub 2012 Jun 24.
Abstract
The recovery of phenolic compounds of Eugenia pyriformis using different solvents was investigated in this study. The compounds were identified and quantified by reverse-phase high-performance liquid chromatography coupled with ultraviolet-visible diode-array detector (RP-HPLC-DAD/UV-vis). Absolute methanol was the most effective extraction agent of phenolic acids and flavonols (588.31 mg/Kg) from Eugenia pyriformis, although similar results (p ≤ 0.05) were observed using methanol/water (1:1 ratio). Our results clearly showed that higher contents of phenolic compounds were not obtained either with the most or the least polar solvents used. Several phenolic compounds were identified in the samples whereas gallic acid and quercetin were the major compounds recovered.
摘要
本研究考察了不同溶剂对番石榴中酚类化合物的提取效果。采用反相高效液相色谱-紫外可见二极管阵列检测法（RP-HPLC-DAD/UV-vis）对化合物进行定性和定量分析。结果表明，对于番石榴中的酚酸和黄酮醇，绝对甲醇（588.31 mg/Kg）是最有效的提取剂，甲醇/水（1:1 比例）也得到了类似的结果（p≤0.05）。我们的研究结果清楚地表明，用最极性或非极性的溶剂提取时，均未获得最高含量的酚类化合物。在样品中鉴定出了几种酚类化合物，其中没食子酸和槲皮素是主要的回收化合物。
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