Xu S X, Wang N Q, Dong Z W
Yao Xue Xue Bao. 1989;24(6):401-6.
Hematoporphyrin derivative (HPD) is a photosensitizer for use in photodynamic therapy (PDT). In this paper, HPD was conjugated with monoclonal antibodies 3G9 or 3H11 for use against gastric cancer in order to enhance the photosensitive effect and reduce side effect of PDT. The biological activities of the McAb conjugates were demonstrated. The killing effect on BGC-823 cells of 3G9-HPD or 3H11-HPD conjugates plus exposure to light showed 17 fold and 8.6 fold greater cytotoxicity, respectively than free HPD at an equivalent HPD concentration in vitro. When 3G9-HPD and 3H11-HPD were used in combination at 7:3, 5:5 and 3:7 proportion, the cytotoxicity was increased 12.9, 11.8 and 9.4 fold on target cells, respectively. The results indicate that the cytotoxicity was not further enhanced by the combination scheme. When tumor-bearing nude mice were treated with the different conjugates. Significant inhibition of tumor growth was observed and the survival period of animals was markedly prolonged in comparison with PBS, free HPD and NIgG-HPD treated groups.
血卟啉衍生物（HPD）是一种用于光动力疗法（PDT）的光敏剂。在本文中，HPD 与单克隆抗体 3G9 或 3H11 偶联，用于对抗胃癌，以增强光动力疗法的光敏效果并降低其副作用。证实了单克隆抗体偶联物的生物学活性。在体外同等 HPD 浓度下，3G9-HPD 或 3H11-HPD 偶联物加光照对 BGC-823 细胞的杀伤作用分别比游离 HPD 的细胞毒性大 17 倍和 8.6 倍。当 3G9-HPD 和 3H11-HPD 以 7:3、5:5 和 3:7 的比例联合使用时，对靶细胞的细胞毒性分别增加了 12.9 倍、11.8 倍和 9.4 倍。结果表明联合方案并未进一步增强细胞毒性。当用不同的偶联物处理荷瘤裸鼠时，与 PBS、游离 HPD 和 NIgG-HPD 处理组相比，观察到肿瘤生长受到显著抑制，动物的生存期明显延长。
