Gomez-Lopez Nardhy, Tong Wing-Chiu, Arenas-Hernandez Marcia, Tanaka Satomi, Hajar Omar, Olson David M, Taggart Michael J, Mitchell Bryan F
Department of Obstetrics and Gynecology, Perinatology Research Branch, Wayne State University, Detroit, MI, USA.
Am J Reprod Immunol. 2015 Apr;73(4):341-52. doi: 10.1111/aji.12333. Epub 2014 Oct 20.
Is increased leukocyte chemotactic activity (CA) from gestational tissues necessary for term or preterm labor in guinea pigs?
Tissue extracts were prepared from pregnant guinea pig decidua-myometrium, cervix, fetal membranes (amniochorion), and placenta during early third trimester (n = 8), term not in labor (TNL, n = 5), and term spontaneous labor (TL, n = 6), RU486-induced preterm labor (PTL, n = 6), or controls (cPTL, n = 5). Leukocyte CA was assessed using a modified Boyden chamber assay. Extract chemokine and maternal progesterone concentrations were quantified by enzyme immunoassay.
Only the extracts from amniochorion demonstrated increased CA through late gestation and labor. In contrast, CA was decreased in extracts from amniochorion and cervix from animals after RU486-induced PTL. Maternal progesterone concentrations remained high in all groups.
Leukocyte CA of intrauterine tissues is increased in term spontaneous labor. However, RU486-induced preterm labor occurs in the absence of increased CA.
豚鼠足月分娩或早产时，妊娠组织中白细胞趋化活性（CA）增加是否必要？
制备孕晚期（n = 8）、足月未临产（TNL，n = 5）、足月自然分娩（TL，n = 6）、RU486诱导的早产（PTL，n = 6）或对照组（cPTL，n = 5）的妊娠豚鼠蜕膜-子宫肌层、宫颈、胎膜（羊膜绒毛膜）和胎盘的组织提取物。使用改良的Boyden小室分析法评估白细胞CA。通过酶免疫测定法定量提取物趋化因子和母体孕酮浓度。
只有羊膜绒毛膜提取物在妊娠晚期和分娩时显示CA增加。相反，RU486诱导的早产动物的羊膜绒毛膜和宫颈提取物中的CA降低。所有组母体孕酮浓度均保持较高水平。
足月自然分娩时子宫内组织的白细胞CA增加。然而，RU486诱导的早产在CA未增加的情况下发生。
