Liu Shikai, Yao Jun, Zhang Jiaren, Liu Zhanjiang
a The Fish Molecular Genetics and Biotechnology Laboratory, Aquatic Genomics Unit , School of Fisheries, Aquaculture and Aquatic Sciences and Program of Cell and Molecular Biosciences, Auburn University , Auburn , AL , USA.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 May;27(3):1963-4. doi: 10.3109/19401736.2014.971308. Epub 2014 Oct 20.
Striped raphael catfish, Platydoras armatulus, belongs to catfish family Doradidae, which are endemic to South America and featured by a single row of thorny scutes along the lateral line. In this study, we determined the complete mitochondrial genome of P. armatulus by next generation sequencing of genomic DNA without prior sample processing or primer design. Bioinformatics analysis provided the full-length mitogenome sequence of 16,470 bp. The P. armatulus mitogenome is composed of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes and 1 control region, showing typical circular molecule structure as in other vertebrates. The whole genome base composition was estimated to be 30.9% A, 25.2% T, 15.9% G and 28.0% C, with A/T bias of 56.1%. The generation of P. armatulus mitogenome sequence enriched catfish molecular resources that are valuable for phylogenetic analysis and species identification studies.
条纹拉斐尔鲶鱼（Platydoras armatulus）属于鲶鱼科（Doradidae），原产于南美洲，其特征是沿着侧线有一排带刺的鳞片。在本研究中,我们通过对基因组DNA进行二代测序测定了条纹拉斐尔鲶鱼的完整线粒体基因组，无需事先进行样本处理或引物设计。生物信息学分析得出全长为16,470bp的线粒体基因组序列。条纹拉斐尔鲶鱼的线粒体基因组由13个蛋白质编码基因、22个tRNA基因、2个rRNA基因和1个控制区组成，呈现出与其他脊椎动物一样典型的环状分子结构。整个基因组的碱基组成为：A占30.9%、T占25.2%、G占15.9%、C占28.0%，A/T偏倚率为56.1%。条纹拉斐尔鲶鱼线粒体基因组序列的产生丰富了鲶鱼分子资源，这对于系统发育分析和物种鉴定研究具有重要价值。
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