Liu Shikai, Zhang Jiaren, Yao Jun, Liu Zhanjiang
a The Fish Molecular Genetics and Biotechnology Laboratory, Aquatic Genomics Unit , School of Fisheries, Aquaculture and Aquatic Sciences and Program of Cell and Molecular Biosciences, Auburn University , Auburn , AL , USA.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 May;27(3):1908-9. doi: 10.3109/19401736.2014.971281. Epub 2014 Oct 20.
The complete mitochondrial genome of the armored catfish, Hypostomus plecostomus, was determined by next generation sequencing of genomic DNA without prior sample processing or primer design. Bioinformatics analysis resulted in the entire mitochondrial genome sequence with length of 16,523 bp. The H. plecostomus mitochondrial genome is consisted of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and 1 control region, showing typical circular molecule structure of mitochondrial genome as in other vertebrates. The whole genome base composition was estimated to be 31.8% A, 27.0% T, 14.6% G, and 26.6% C, with A/T bias of 58.8%. This work provided the H. plecostomus mitochondrial genome sequence which should be valuable for species identification, phylogenetic analysis and conservation genetics studies in catfishes.
通过对基因组DNA进行下一代测序，在没有事先样本处理或引物设计的情况下，确定了甲鲶Hypostomus plecostomus的完整线粒体基因组。生物信息学分析得到了长度为16,523 bp的完整线粒体基因组序列。Hypostomus plecostomus线粒体基因组由13个蛋白质编码基因、22个tRNA基因、2个rRNA基因和1个控制区组成，呈现出与其他脊椎动物线粒体基因组典型的环状分子结构。全基因组碱基组成估计为A占31.8%、T占27.0%、G占14.6%、C占26.6%，A/T偏倚率为58.8%。这项工作提供了Hypostomus plecostomus线粒体基因组序列，这对于鲶鱼的物种鉴定、系统发育分析和保护遗传学研究应该是有价值的。
