Hu Chaochao, Yu Tingting, Xie Wenli, Zhu Ya, Chang Qing
a School of Life Science, Nanjing Normal University , Nanjing , Jiangsu , P. R. China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 May;27(3):1936-7. doi: 10.3109/19401736.2014.971295. Epub 2014 Oct 20.
The northern lapwing, Vanellus vanellus (Charadriiformes: Charadriidae), is commonly found in temperate Eurasia. In this study, the complete mitogenome sequence of V. vanellus has been determined by polymerase chain reaction (PCR) method using 13 primer pairs. It was a circular molecule with 16,795 bp in length which contained 13 protein-coding genes, 2 ribosomal RNAs, 22 transfer RNAs and a control region. The composition and gene order are similar to most other vertebrates. There are 28 genes encoded on the H-strand, 9 genes (ND6 subunit and 8 tRNA genes) encoded on the L-strand. The overall base composition of the H-strand is A (31.44%), T (24.03%), G (13.76%), C (30.77%), with a slight A + T bias of 55.47%. This mitogenome sequence of V. vanellus could contribute to a better solution of its phylogenetic position and will contribute to further genetic researches on Charadriidae.
凤头麦鸡(Vanellus vanellus,鸻形目:鸻科)常见于温带欧亚大陆。在本研究中,利用13对引物通过聚合酶链式反应(PCR)方法测定了凤头麦鸡的完整线粒体基因组序列。它是一个长度为16,795 bp的环状分子,包含13个蛋白质编码基因、2个核糖体RNA、22个转运RNA和一个控制区。其组成和基因顺序与大多数其他脊椎动物相似。H链上编码28个基因,L链上编码9个基因(ND6亚基和8个tRNA基因)。H链的总体碱基组成为A(31.44%)、T(24.03%)、G(13.76%)、C(30.77%),A+T略有偏向,为55.47%。凤头麦鸡的这个线粒体基因组序列有助于更好地确定其系统发育位置,并将有助于对鸻科进行进一步的遗传学研究。