She Huishang, Zhao Guanghong, Zhou Lizhi, Gu Changming
a School of Resources and Environmental Engineering, Anhui University , Hefei , P.R. China .
b Anhui Biodiversity Information Center , Hefei , P.R. China , and.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Sep;27(5):3120-1. doi: 10.3109/19401736.2015.1007293. Epub 2015 Mar 11.
The Grey-headed Lapwing (Vanellus cinereus) is a Vanellus bird belonging to the group Ciconiiformes, which breeds in northeast China and Japan. In this study, we sequenced its complete mitochondrial genome by PCR-based method. The mitochondrial DNA is packaged in a compact 17,135 based pair (bp) circular molecule with A + T content of 55.14%. It contains 37 typical mitochondrial genes, including 13 protein-coding genes, 2 rRNAs and 22 tRNAs and a non-coding control region (D-loop). All protein-coding genes are initiated by ATG codon, except for the COI gene and ND5 gene starting with GTG codon, and ND3 uses ATC codon. TAA is the most frequent stop codon. All tRNAs possess the classic cloverleaf secondary structure except for tRNA(Ser(AGY)) and tRNA(Leu(CUN)), which lack the ''DHU'' stem. The D-loop is a 1563 bp long A + T-rich region, which is located between tRNA(Glu) and tRNA(Phe).
灰头麦鸡（Vanellus cinereus）是鸻形目麦鸡属的一种鸟类，在中国东北和日本繁殖。在本研究中，我们采用基于聚合酶链式反应（PCR）的方法对其完整线粒体基因组进行了测序。线粒体DNA被包装在一个紧凑的17,135碱基对（bp）的环状分子中，A+T含量为55.14%。它包含37个典型的线粒体基因，包括13个蛋白质编码基因、2个核糖体RNA（rRNA）和22个转运RNA（tRNA）以及一个非编码控制区（D-loop）。除细胞色素氧化酶亚基I（COI）基因和NADH脱氢酶亚基5（ND5）基因起始密码子为GTG，ND3基因起始密码子为ATC外，所有蛋白质编码基因均以ATG起始密码子开始。TAA是最常见的终止密码子。除了缺少“DHU”茎的tRNA（Ser(AGY)）和tRNA（Leu(CUN)）外，所有tRNA都具有经典的三叶草二级结构。D-loop是一个1563bp长的富含A+T的区域，位于tRNA（Glu）和tRNA（Phe）之间。
