Department of Biomedical Engineering, The Pennsylvania State University, University Park, PA, 16802, USA.
Center for Molecular Immunology and Infectious Disease, Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA, 16802, USA.
Macromol Biosci. 2017 Nov;17(11). doi: 10.1002/mabi.201700201. Epub 2017 Aug 15.
Synthetic molecular libraries hold great potential to advance the biomaterial development. However, little effort is made to integrate molecules with molecular recognition abilities selected from different libraries into a single biomolecular material. The purpose of this work is to incorporate peptides and nucleic acid aptamers into a porous hydrogel to develop a dual-functional biomaterial. The data show that an anti-integrin peptide can promote the attachment and growth of endothelial cells in a 3D porous poly(ethylene glycol) hydrogel and an antivascular endothelial growth factor aptamer can sequester and release VEGF of high bioactivity. Importantly, the dual-functional porous hydrogel enhances the growth and survival of endothelial cells. This work demonstrates that molecules selected from different synthetic libraries can be integrated into one system for the development of novel biomaterials.
合成分子文库在推进生物材料发展方面具有巨大潜力。然而，将不同文库中具有分子识别能力的分子整合到单个生物分子材料中这方面的工作却很少有人涉及。本工作的目的是将肽和核酸适体整合到多孔水凝胶中，以开发一种双重功能的生物材料。数据表明，一种抗整合素肽可促进内皮细胞在 3D 多孔聚（乙二醇）水凝胶中的附着和生长，而一种抗血管内皮生长因子适体可隔离和释放具有高生物活性的 VEGF。重要的是，双重功能的多孔水凝胶可增强内皮细胞的生长和存活。本工作证明了可以将从不同合成文库中选择的分子整合到一个系统中，以开发新型生物材料。
