Schulze Alexandra E, de Beer Dalene, de Villiers André, Manley Marena, Joubert Elizabeth
Department of Food Science and ‡Department of Chemistry and Polymer Science, Stellenbosch University , Private Bag X1, Matieland (Stellenbosch) 7602, South Africa.
J Agric Food Chem. 2014 Oct 29;62(43):10542-51. doi: 10.1021/jf5028735. Epub 2014 Oct 20.
Cyclopia species are used for the production of honeybush tea and food ingredient extracts associated with many health benefits. A species-specific high-performance liquid chromatography (HPLC) method for Cyclopia maculata, developed and validated, allowed quantification of the major compounds in extracts from "unfermented" and fermented C. maculata. Two xanthones were tentatively identified for the first time in a Cyclopia species, whereas an additional four compounds were tentatively identified for the first time in C. maculata. "Fermentation" (oxidation) decreased the content of all compounds, with the exception of vicenin-2. Similarity analysis of the chromatographic fingerprints of unfermented C. maculata aqueous extracts showed extremely low variation (r ≥ 0.97) between samples. Some differences between wild-harvested and cultivated seedling plants were, however, demonstrated using principal component analysis. Quantitative data of selected compounds confirmed the low level of variation, making this Cyclopia species ideal for the production of standardized food ingredient extracts.
独眼茶属植物被用于生产蜜树茶以及具有多种健康益处的食品成分提取物。一种针对黄斑独眼茶开发并验证的物种特异性高效液相色谱（HPLC）方法，能够对“未发酵”和发酵的黄斑独眼茶提取物中的主要化合物进行定量分析。首次在独眼茶属植物中初步鉴定出两种氧杂蒽酮，而在黄斑独眼茶中首次初步鉴定出另外四种化合物。“发酵”（氧化）降低了除异荭草苷-2之外所有化合物的含量。未发酵的黄斑独眼茶水提取物色谱指纹图谱的相似性分析表明，样品之间的差异极小（r≥0.97）。然而，通过主成分分析显示，野生采集的植株和栽培幼苗植株之间存在一些差异。所选化合物的定量数据证实了变异水平较低，这使得这种独眼茶属植物成为生产标准化食品成分提取物的理想选择。
