Zhao Guo Dong, Sun Rui, Ni Shi Lei, Xia Qiang
State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University , Nanjing , PR China .
J Microencapsul. 2015;32(2):157-65. doi: 10.3109/02652048.2014.973072. Epub 2014 Oct 20.
This article describes the physicochemical properties of chitosan-coated liposomes containing skin-protecting agents, coenzyme Q10 and alpha-lipoic acid (CCAL). CCAL had a spherical shell-core structure and liposomes inverted the surface charge from negative to positive after coating with chitosan. Compared with the uncoated liposome, CCAL had higher zeta potential, larger droplet size and long-term stability. Fourier transform infrared spectroscopy (FTIR) study showed that the driving force for chitosan coating the liposomes was enhanced via hydrogen bonding and ionic bond force between the chitosan and the alpha-lipoic acid. While the encapsulation efficiency (EE) of alpha-lipoic acid also increased by interacting with the chitosan shell. In vitro antioxidant activity study showed an excellent hydroxyl radical scavenging activity of CCAL. In vitro release study displayed a sustained drug release, and in vitro penetration studies promoted the accumulation of drugs in rabbit skin.
本文描述了含有皮肤保护剂辅酶Q10和α-硫辛酸的壳聚糖包被脂质体（CCAL）的物理化学性质。CCAL具有球形的核壳结构，用壳聚糖包被后脂质体表面电荷从负变为正。与未包被的脂质体相比，CCAL具有更高的zeta电位、更大的液滴尺寸和长期稳定性。傅里叶变换红外光谱（FTIR）研究表明，壳聚糖包被脂质体的驱动力是通过壳聚糖与α-硫辛酸之间的氢键和离子键力增强的。而α-硫辛酸的包封率（EE）也通过与壳聚糖壳相互作用而增加。体外抗氧化活性研究表明CCAL具有优异的羟基自由基清除活性。体外释放研究显示药物持续释放，体外渗透研究促进了药物在兔皮肤中的积累。
