Zhao Guodong, Hu Caibiao, Xue Ying
Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, Jiangsu, China.
State Key Laboratory of Bioelectronics, School of Biological Science & Medical Engineering, Southeast University, Nanjing, Jiangsu, China.
J Cosmet Dermatol. 2018 Apr;17(2):258-262. doi: 10.1111/jocd.12369. Epub 2017 Jul 19.
Chitosan-coated liposome containing both coenzyme Q10 and alpha-lipoic acid (CCAL) was a novel multifunctional nano delivery system exhibited long-term stability at room temperature and high encapsulation efficiency. Which containing two kinds of antioxidants (coenzyme Q10 and alpha-lipoic acid) and two antibacterial agents (chitosan and alpha-lipoic acid). However, the further biocompatibility, such as cytotoxicity and antioxidant activity, and antimicrobial activity of CCAL, has not been systematically evaluated.
This study aims to evaluate the cytotoxicity, antioxidant activity and antimicrobial activity of CCAL.
This article evaluated the in vitro cytotoxicity and antioxidant activity of CCAL by CCK8 assay, and antimicrobial activity on Staphylococcus aureus and Escherichia coli was investigated.
For antioxidant activity study, CCAL displayed significant antioxidant effect when compared with control group (P<.01). Finally, CCAL also exhibited antimicrobial activity, and CCAL showed stronger bactericidal effect with Gram-positive bacteria than Gram-negative bacteria.
CCAL could be a potential antioxidant delivery system for cosmetics or pharmaceuticals without cytotoxicity and artificial preservatives free.
含有辅酶Q10和α-硫辛酸的壳聚糖包被脂质体（CCAL）是一种新型多功能纳米递送系统，在室温下具有长期稳定性且包封率高。它含有两种抗氧化剂（辅酶Q10和α-硫辛酸）和两种抗菌剂（壳聚糖和α-硫辛酸）。然而，CCAL的进一步生物相容性，如细胞毒性和抗氧化活性以及抗菌活性，尚未得到系统评估。
本研究旨在评估CCAL的细胞毒性、抗氧化活性和抗菌活性。
本文通过CCK8法评估CCAL的体外细胞毒性和抗氧化活性，并研究其对金黄色葡萄球菌和大肠杆菌的抗菌活性。
在抗氧化活性研究中，与对照组相比，CCAL表现出显著的抗氧化作用（P<0.01）。最后，CCAL也表现出抗菌活性，且CCAL对革兰氏阳性菌的杀菌效果强于革兰氏阴性菌。
CCAL可能是一种潜在的抗氧化剂递送系统，可用于化妆品或药品，无细胞毒性且不含人工防腐剂。
