Rossi Jean-Christophe, Maret Barbara, Vidot Kevin, Francoia Jean-Patrick, Cangiotti Michela, Lucchi Susanna, Coppola Concetta, Ottaviani Maria Francesca
Institut des Biomolecules Max Mousseron/UMR 5247 Université Montpellier II, Place Eugène Bataillon, 34095, Montpellier, France.
Macromol Biosci. 2015 Feb;15(2):275-90. doi: 10.1002/mabi.201400341. Epub 2014 Oct 20.
Poly-L-lysine is a biocompatible polymer used for drug or gene delivery, for transport through cellular membranes, and as nanosized magnetic resonance imaging contrast agents. Cu(II)-poly-L-lysine complexes are of particular interest for their role in biocatalysis. In this study, poly-L-lysine dendrigrafts (DGLs) at different generations (G2, G3, and G4) are synthesized and characterized in absence and presence of Cu(II) by means of electron paramagnetic resonance (EPR), UV-Vis, potentiometric titration and circular dichroism (CD). The analysis is performed as a function of the [Cu(II)]/[Lys] (=R) molar ratio, pH and generation by identifying differently flexible complexes in different dendrimer regions. The amine sites in the lateral chains become increasingly involved with the increase of pH. The good agreement and complementarity of the results from the different techniques provide an integrate view of the structural and dynamic properties of Cu(II)-DGL complexes implementing their use as biocatalysts.
聚-L-赖氨酸是一种生物相容性聚合物，用于药物或基因递送、穿过细胞膜的运输以及作为纳米级磁共振成像造影剂。铜（II）-聚-L-赖氨酸配合物因其在生物催化中的作用而备受关注。在本研究中，通过电子顺磁共振（EPR）、紫外可见光谱（UV-Vis）、电位滴定和圆二色性（CD）等手段，对不同代数（G2、G3和G4）的聚-L-赖氨酸树枝状接枝物（DGLs）在有无铜（II）的情况下进行了合成和表征。分析是作为[铜（II）]/[赖氨酸]（=R）摩尔比、pH值和代数的函数进行的，通过识别不同树枝状聚合物区域中具有不同柔韧性的配合物来实现。随着pH值的升高，侧链中的胺位点越来越多地参与其中。不同技术结果的良好一致性和互补性提供了铜（II）-DGL配合物结构和动态性质的综合视图，从而实现了它们作为生物催化剂的应用。
