Levanon D, Danciger E, Dafni N, Bernstein Y, Elson A, Moens W, Brandeis M, Groner Y
Department of Virology, Weizmann Institute of Science, Rehovot, Israel.
DNA. 1989 Dec;8(10):733-43. doi: 10.1089/dna.1989.8.733.
The complete mRNA sequence of the human liver-type phosphofructokinase (hPFKL) was determined. The sequence included 55 nucleotides of 5' and 515 of 3' noncoding regions, as well as 2,337 nucleotides encoding the 779 amino acids of the hPFKL. Extensive similarity (approximately 90%) in the coding region was observed between the hPFKL and the mouse PFKL, whereas the degree of similarity between different types of PFK, i.e., hPFKL and human muscle-type PFK (hPFKM), was merely 68%. Nevertheless, striking similarity between these different types of PFK was noticed when the amino acid residues creating the various active sites of the enzyme were compared. Human PFK L- and M-specific probes were constructed and used to quantitate the mRNA levels in fetal and adult brains and fetal liver. It was found that while relative amount of PFKL mRNA in adult brain was one-fourth of that detected in fetal brain the level of PFKM mRNA in adult brain was slightly higher than in fetal tissue, suggesting that PFK expression might be controlled at the transcriptional level.
测定了人肝脏型磷酸果糖激酶（hPFKL）的完整mRNA序列。该序列包括5'端的55个核苷酸和3'端的515个非编码区核苷酸，以及编码hPFKL的779个氨基酸的2337个核苷酸。在hPFKL和小鼠PFKL的编码区观察到广泛的相似性（约90%），而不同类型的PFK，即hPFKL和人肌肉型PFK（hPFKM）之间的相似程度仅为68%。然而，当比较构成酶的各种活性位点的氨基酸残基时，注意到这些不同类型的PFK之间存在显著相似性。构建了人PFK L和M特异性探针，并用于定量胎儿和成人脑以及胎儿肝脏中的mRNA水平。结果发现，虽然成人大脑中PFKL mRNA的相对量是胎儿大脑中检测到的量的四分之一，但成人大脑中PFKM mRNA的水平略高于胎儿组织，这表明PFK的表达可能在转录水平受到调控。
