Arciniegas Enrique, Carrillo Luz Marina, Páez Erika, Rojas Héctor, Ramírez Richard, Reales Eysi, Chopite Marina
Institute of Biomedicine "Dr. Jacinto Convit", and Service Autonomous Institute of Biomedicine, Central University of Venezuela, Caracas, Venezuela.
Service Autonomous Institute of Biomedicine, Central University of Venezuela, Caracas, Venezuela.
Histol Histopathol. 2015 Apr;30(4):453-63. doi: 10.14670/HH-30.453. Epub 2014 Oct 21.
Mucin 1 (MUC1) is a transmembrane glycoprotein that protects epithelial cells from injury caused by external stimuli. In addition to this role, MUC1 is involved in cell-cell adhesion, proliferation, motility, invasion and survival. In epithelial cells, MUC1 expression is regulated by binding of TNFα to TNFR1 and activation of the NFκB pathway. In human skin, MUC1 is not expressed in normal epidermis but rather in pre-malignant and malignant conditions. Nevertheless, the expression of MUC1 and its implication in psoriasis vulgaris has not been considered. Here, we show that MUC1 was present in the epidermis of psoriatic plaques observed in 11 biopsies from patients diagnosed with psoriasis vulgaris which were compared with 5 normal human skin. Interestingly, MUC1 in addition to being localized at the apical surface of some suprabasal keratinocytes, was also localized over the entire cell surface of some of these cells and some basal keratinocytes. Conversely, no MUC1 immunoreactivity was detected in the epidermis of normal skin. Additionally, we demonstrated that activated TNFR1, c-Src, IKKα/β and p50/p65 were present in the epidermal thickening. This study demonstrates the presence of MUC1 in psoriatic plaque and suggests a possible role for MUC1 during the motility, migration and survival of human keratinocytes, where activated TNFR1, c-Src and NFκB seem to be required.
黏蛋白1（MUC1）是一种跨膜糖蛋白，可保护上皮细胞免受外部刺激造成的损伤。除了这一作用外，MUC1还参与细胞间黏附、增殖、运动、侵袭和存活。在上皮细胞中，MUC1的表达受肿瘤坏死因子α（TNFα）与肿瘤坏死因子受体1（TNFR1）结合以及核因子κB（NFκB）信号通路激活的调控。在人类皮肤中，MUC1在正常表皮中不表达，而是在癌前和恶性病变中表达。然而，MUC1的表达及其在寻常型银屑病中的意义尚未得到研究。在此，我们发现，在11例寻常型银屑病患者活检的银屑病斑块表皮中存在MUC1，并与5例正常人类皮肤进行了比较。有趣的是，MUC1除了定位于一些基底层上角质形成细胞的顶端表面外，还定位于其中一些细胞和一些基底角质形成细胞完整的细胞表面。相反，在正常皮肤的表皮中未检测到MUC1免疫反应性。此外，我们还证明，活化的TNFR1、c-Src、IKKα/β和p50/p65存在于表皮增厚处。本研究证明了MUC1在银屑病斑块中的存在，并提示MUC1在人类角质形成细胞的运动、迁移和存活过程中可能发挥作用，其中活化的TNFR1、c-Src和NFκB似乎是必需的。
