白斑综合征病毒VP52B的特性及其与VP26的相互作用

Characterization of white spot syndrome virus VP52B and its interaction with VP26.

作者信息

Lin Fanyu, Jie Zuliang, Hou Luhong, Li Fang, Yang Feng

机构信息

State Key Laboratory Breeding Base of Marine Genetic Resources, Key Laboratory of Marine Genetic Resources of State Oceanic Administration, Third Institute of Oceanography, Xiamen, 361005, People's Republic of China.

出版信息

Virus Genes. 2015 Feb;50(1):46-51. doi: 10.1007/s11262-014-1126-0. Epub 2014 Oct 21.

DOI:10.1007/s11262-014-1126-0

PMID:25331340

Abstract

White spot syndrome virus (WSSV) is one of the major pathogens of cultured shrimp. Identification of envelope protein interactions has become a central issue for the understanding of WSSV assembly. In this paper, WSSV envelope protein VP52B was fused with GST-tag and expressed in Escherichia coli BL-21(DE3). Immunogold-electron microscopy revealed that VP52B was located on the outside surface of WSSV virions. Far-Western blotting analysis suggested that VP52B might directly interact with a major viral envelope protein VP26, and their interaction was confirmed by GST pull-down assay. Further investigation showed that the VP52B binding domain was located between residues 135-170 of VP26. These findings will enhance our understanding of the molecular mechanisms of WSSV morphogenesis.

摘要

白斑综合征病毒（WSSV）是养殖虾的主要病原体之一。包膜蛋白相互作用的鉴定已成为理解WSSV组装的核心问题。本文将WSSV包膜蛋白VP52B与GST标签融合，并在大肠杆菌BL-21(DE3)中表达。免疫金电子显微镜显示VP52B位于WSSV病毒粒子的外表面。Far-Western印迹分析表明VP52B可能直接与主要病毒包膜蛋白VP26相互作用，并且通过GST下拉试验证实了它们的相互作用。进一步研究表明，VP52B结合结构域位于VP26的135-170位氨基酸残基之间。这些发现将增进我们对WSSV形态发生分子机制的理解。