白斑综合征病毒的VP26通过与VP51结合，作为病毒粒子包膜与核衣壳之间的连接蛋白发挥作用。
VP26 of white spot syndrome virus functions as a linker protein between the envelope and nucleocapsid of virions by binding with VP51.
作者信息
Wan Qiang, Xu Limei, Yang Feng
机构信息
School of Life Sciences, Xiamen University, Xiamen 361005, People's Republic of China.
出版信息
J Virol. 2008 Dec;82(24):12598-601. doi: 10.1128/JVI.01732-08. Epub 2008 Oct 8.
Abstract
The envelopment of the nucleocapsid is an important step in white spot syndrome virus (WSSV) assembly. Previous studies showed that VP26, a major envelope protein of WSSV, can interact with viral nucleocapsid. In this study, using the biotin label transfer technique, we found that the biotin label was transferred from Bio-rVP26 to the viral capsid protein VP51 or from Bio-MBP-VP51 to VP26. Far-Western analyses provided further evidence for direct interaction between VP26 and VP51. Therefore, we conclude that VP26 functions as a matrix-like linker protein between the viral envelope and nucleocapsid, which suggests that VP26 is a key factor in the envelopment of WSSV virion.
摘要
核衣壳的包裹是白斑综合征病毒（WSSV）组装过程中的一个重要步骤。先前的研究表明，WSSV的主要包膜蛋白VP26可与病毒核衣壳相互作用。在本研究中，利用生物素标记转移技术，我们发现生物素标记从生物素化的重组VP26（Bio-rVP26）转移至病毒衣壳蛋白VP51，或从生物素化的麦芽糖结合蛋白-VP51（Bio-MBP-VP51）转移至VP26。蛋白质免疫印迹法进一步证明了VP26与VP51之间存在直接相互作用。因此，我们得出结论，VP26作为病毒包膜与核衣壳之间类似基质的连接蛋白发挥作用，这表明VP26是WSSV病毒粒子包裹过程中的关键因子。
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