Wan Qiang, Xu Limei, Yang Feng
School of Life Sciences, Xiamen University, Xiamen 361005, People's Republic of China.
J Virol. 2008 Dec;82(24):12598-601. doi: 10.1128/JVI.01732-08. Epub 2008 Oct 8.
The envelopment of the nucleocapsid is an important step in white spot syndrome virus (WSSV) assembly. Previous studies showed that VP26, a major envelope protein of WSSV, can interact with viral nucleocapsid. In this study, using the biotin label transfer technique, we found that the biotin label was transferred from Bio-rVP26 to the viral capsid protein VP51 or from Bio-MBP-VP51 to VP26. Far-Western analyses provided further evidence for direct interaction between VP26 and VP51. Therefore, we conclude that VP26 functions as a matrix-like linker protein between the viral envelope and nucleocapsid, which suggests that VP26 is a key factor in the envelopment of WSSV virion.
核衣壳的包裹是白斑综合征病毒（WSSV）组装过程中的一个重要步骤。先前的研究表明，WSSV的主要包膜蛋白VP26可与病毒核衣壳相互作用。在本研究中，利用生物素标记转移技术，我们发现生物素标记从生物素化的重组VP26（Bio-rVP26）转移至病毒衣壳蛋白VP51，或从生物素化的麦芽糖结合蛋白-VP51（Bio-MBP-VP51）转移至VP26。蛋白质免疫印迹法进一步证明了VP26与VP51之间存在直接相互作用。因此，我们得出结论，VP26作为病毒包膜与核衣壳之间类似基质的连接蛋白发挥作用，这表明VP26是WSSV病毒粒子包裹过程中的关键因子。
