Zhou Qing, Xu Limei, Li Hui, Qi Yi-Peng, Yang Feng
State Key Laboratory of Virology, Section of Molecular Virology, College of Life Sciences, Wuhan University, Wuhan, People's Republic of China.
J Virol. 2009 May;83(9):4709-12. doi: 10.1128/JVI.02360-08. Epub 2009 Feb 11.
Early events in white spot syndrome virus (WSSV) morphogenesis, particularly the formation of viral membranes, are poorly understood. The major envelope proteins of WSSV are VP28, VP26, VP24, and VP19. Our previous results indicated that VP28 interacts with VP26 and VP24. In the present study, we used coimmunoprecipitation assays and pull-down assays to confirm that the four major proteins in the WSSV envelope can form a multiprotein complex. Yeast two-hybrid assays were also used to test for interactions among the four proteins. In summary, three pairwise protein interactions (VP19-VP28, VP19-VP24, and VP24-VP26) and one self-association (VP24-VP24) were identified for the first time.
对白斑综合征病毒（WSSV）形态发生的早期事件，尤其是病毒膜的形成，人们了解甚少。WSSV的主要包膜蛋白是VP28、VP26、VP24和VP19。我们之前的结果表明VP28与VP26和VP24相互作用。在本研究中，我们使用了免疫共沉淀分析和下拉分析来证实WSSV包膜中的这四种主要蛋白能够形成多蛋白复合物。酵母双杂交分析也用于检测这四种蛋白之间的相互作用。总之，首次鉴定出了三种两两蛋白相互作用（VP19-VP28、VP19-VP24和VP24-VP26）以及一种自身缔合（VP24-VP24）。
