Yin Zhaozheng, Su Hang, Ker Elmer, Li Mingzhong, Li Haohan
Med Image Comput Comput Assist Interv. 2014;17(Pt 1):41-8. doi: 10.1007/978-3-319-10404-1_6.
We propose a novel cell segmentation approach by estimating a cell-sensitive camera response function based on variously exposed phase contrast microscopy images on the same cell dish. Using the cell-sensitive microscopy imaging, cells' original irradiance signals are restored from all exposures and the irradiance signals on non-cell background regions are restored as a uniform constant (i.e., the imaging system is sensitive to cells only but insensitive to non-cell background). Cell segmentation is then performed on the restored irradiance signals by simple thresholding. The experimental results validate that high quality cell segmentation can be achieved by our approach.
我们提出了一种新颖的细胞分割方法，通过基于同一细胞培养皿上不同曝光的相差显微镜图像估计细胞敏感的相机响应函数。利用细胞敏感的显微镜成像，从所有曝光中恢复细胞的原始辐照度信号，并且将非细胞背景区域上的辐照度信号恢复为均匀常数（即成像系统仅对细胞敏感，而对非细胞背景不敏感）。然后通过简单的阈值处理对恢复的辐照度信号进行细胞分割。实验结果验证了我们的方法能够实现高质量的细胞分割。
