Department of Radiology, Drum Tower Hospital, School of Medicine, Nanjing University , Nanjing, Jiangsu, P. R. China 210093.
ACS Appl Mater Interfaces. 2014 Dec 10;6(23):21316-24. doi: 10.1021/am5061933. Epub 2014 Nov 3.
Three kinds of amphiphilic copolymer, that is, poly(ε-caprolactone)-SS-poly(ethylene glycol) (PCL-SS-PEG), poly(ε-caprolactone)-polyethylenimine (PCL-PEI), and poly(ε-caprolactone)-polyethylenimine-folate (PCL-PEI-Fol) were synthesized and self-assembled into surface engineered hybrid nanoparticles (NPs). Morphological studies elucidated the stable, spherical, and uniform sandwich structure of the NPs. PCL-PEI and PCL-SS-PEG segments have introduced pH and reduction responsive characteristics in these NPs, while PCL-PEI-FA copolymers could provide specific targeting capability to cancer cells. The stimuli responsive capabilities of these NPs were carried out. Negative-to-positive charge reversible property, in response to the pH change, was investigated by zeta potential and nuclear magnetic resonance (NMR) measurements. The structure cleavage, due to redox gradient, was studied by dynamic light scattering (DLS) and transmission electron microscopy (TEM). These NPs showed controlled degradation, better drug release, less toxicity, and effective uptake in MCF-7 breast cancer cells. These multifunctional NPs showed promising potential in the treatment of cancer.
三种两亲性共聚物，即聚（ε-己内酯）-SS-聚（乙二醇）（PCL-SS-PEG）、聚（ε-己内酯）-聚乙烯亚胺（PCL-PEI）和聚（ε-己内酯）-聚乙烯亚胺-叶酸（PCL-PEI-Fol）被合成并自组装成表面工程化的混合纳米颗粒（NPs）。形态学研究阐明了 NPs 的稳定、球形和均匀的三明治结构。PCL-PEI 和 PCL-SS-PEG 段在这些 NPs 中引入了 pH 和还原响应特性，而 PCL-PEI-FA 共聚物可以为癌细胞提供特异性靶向能力。这些 NPs 的刺激响应能力得到了研究。通过zeta 电位和核磁共振（NMR）测量研究了负到正电荷可逆性质，以响应 pH 值的变化。通过动态光散射（DLS）和透射电子显微镜（TEM）研究了由于氧化还原梯度引起的结构裂解。这些 NPs 表现出可控降解、更好的药物释放、更低的毒性和在 MCF-7 乳腺癌细胞中的有效摄取。这些多功能 NPs 在癌症治疗中显示出有前途的潜力。
