Torres-Martin Miguel, Lassaletta Luis, Isla Alberto, De Campos Jose M, Pinto Giovanny R, Burbano Rommel R, Castresana Javier S, Melendez Barbara, Rey Juan A
Molecular Neuro-Oncogenetics Laboratory, Research Unit, Hospital Universitario La Paz, IdiPAZ, Madrid, Spain.
Department of Otolaryngology, Hospital Universitario La Paz, IdiPAZ, Madrid, Spain.
Oncol Rep. 2014 Dec;32(6):2327-34. doi: 10.3892/or.2014.3526. Epub 2014 Oct 3.
Schwannomas and grade I meningiomas are non‑metastatic neoplasms that share the common mutation of gene NF2. They usually appear in neurofibromatosis type 2 patients. Currently, there is no drug treatment available for both tumors, thus the use of wide expression technologies is crucial to identify therapeutic targets. Affymetrix Human Gene 1.0 ST was used to test global gene expression in 22 meningiomas, 31 schwannomas and, as non-tumoral controls, 3 healthy meningeal tissues, 8 non-tumoral nerves and 1 primary Schwann cell culture. A non-stringent P-value cut-off and fold change were used to establish deregulated genes. We identified a subset of genes that were upregulated in meningiomas and schwannomas when compared to their respectively healthy tissues, including PDGFD, CDH1 and SLIT2. Thus, these genes should be thoroughly studied as targets in a possible combined treatment.
施万细胞瘤和I级脑膜瘤是具有共同的NF2基因突变的非转移性肿瘤。它们通常出现在2型神经纤维瘤病患者中。目前，这两种肿瘤均无药物治疗方法，因此，广泛应用表达技术对于确定治疗靶点至关重要。使用Affymetrix人类基因1.0 ST芯片检测了22例脑膜瘤、31例施万细胞瘤以及作为非肿瘤对照的3例健康脑膜组织、8条非肿瘤神经和1例原代施万细胞培养物中的整体基因表达。采用宽松的P值截止标准和倍数变化来确定失调基因。我们鉴定出了一组与各自健康组织相比在脑膜瘤和施万细胞瘤中上调的基因，包括血小板衍生生长因子D（PDGFD）、钙黏蛋白1（CDH1）和slit同源物2（SLIT2）。因此，这些基因作为联合治疗中的靶点应进行深入研究。
