骨髓间充质干细胞增加损伤纹状体中的多巴胺合成。
Bone marrow-derived mesenchymal stem cells increase dopamine synthesis in the injured striatum.
机构信息
Department of Neurology, Henan Provincial People's Hospital, Zhengzhou 450003, Henan Province, China.
Department of Anatomy, Zhengzhou University, Zhengzhou 450004, Henan Province, China.
出版信息
Neural Regen Res. 2012 Dec 5;7(34):2653-62. doi: 10.3969/j.issn.1673-5374.2012.34.002.
Previous studies showed that tyrosine hydroxylase or neurturin gene-modified cells transplanted into rats with Parkinson's disease significantly improved behavior and increased striatal dopamine content. In the present study, we transplanted tyrosine hydroxylase and neurturin gene-modified bone marrow-derived mesenchymal stem cells into the damaged striatum of Parkinson's disease model rats. Several weeks after cell transplantation, in addition to an improvement of motor function, tyrosine hydroxylase and neurturin proteins were up-regulated in the injured striatum, and importantly, levels of dopamine and its metabolite 3,4-dihydroxyphenylacetic acid increased significantly. Furthermore, the density of the D2 dopamine receptor in the postsynaptic membranes of dopaminergic neurons was decreased. These results indicate that transplantation of tyrosine hydroxylase and neurturin gene-modified bone marrow-derived mesenchymal stem cells increases dopamine synthesis and significantly improves the behavior of rats with Parkinson's disease.
先前的研究表明，将酪氨酸羟化酶或神经营养因子基因修饰的细胞移植到帕金森病大鼠体内，可显著改善行为并增加纹状体多巴胺含量。在本研究中，我们将酪氨酸羟化酶和神经营养因子基因修饰的骨髓间充质干细胞移植到帕金森病模型大鼠的损伤纹状体中。细胞移植数周后，除了运动功能的改善外，损伤纹状体中的酪氨酸羟化酶和神经营养因子蛋白表达上调，更重要的是，多巴胺及其代谢产物 3,4-二羟基苯乙酸的水平显著增加。此外，多巴胺能神经元突触后膜上的 D2 多巴胺受体密度降低。这些结果表明，移植酪氨酸羟化酶和神经营养因子基因修饰的骨髓间充质干细胞可增加多巴胺的合成，并显著改善帕金森病大鼠的行为。
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