Department of Neurology, the First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China.
Department of Neurology, Shenyang Brain Hospital, Shenyang 110001, Liaoning Province, China.
Neural Regen Res. 2012 Dec 5;7(34):2698-704. doi: 10.3969/j.issn.1673-5374.2012.34.007.
We investigated the effects of ipsilateral versus bilateral limb-training on promotion of endogenous neural stem cells in the peripheral infarct zone and the corresponding cerebral region in the unaffected hemisphere of rats with cerebral infarction. Middle cerebral artery occlusion was induced in Wistar rats. The rat forelimb on the unaffected side was either wrapped up with tape to force the use of the paretic forelimb in rats or not braked to allow bilateral forelimbs to participate in training. Daily training consisted of mesh drum training, balance beam training, and stick rolling training for a total of 40 minutes, once per day. Control rats received no training. At 14 days after functional training, rats receiving bilateral limb-training exhibited milder neurological impairment than that in the ipsilateral limb-training group or the control group. The number of nestin/glial fibrillary acidic protein-positive and nestin/microtubule-associated protein 2-positive cells in the peripheral infarct zone and in the corresponding cerebral region in the unaffected hemisphere was significantly higher in rats receiving bilateral limb-training than in rats receiving ipsilateral limb-training. These data suggest that bilateral limb-training can promote the proliferation and differentiation of endogenous neural stem cells in the bilateral hemispheres after cerebral infarction and accelerate the recovery of neurologic function. In addition, bilateral limb-training produces better therapeutic effects than ipsilateral limb-training.
我们研究了同侧和双侧肢体训练对促进脑梗死后梗死区外周和未受影响半球相应脑区内源性神经干细胞的影响。采用大脑中动脉闭塞法制备 Wistar 大鼠脑梗死后模型。将未受影响侧大鼠的前肢用胶带包裹以强迫其使用瘫痪的前肢，或者不制动以允许双侧前肢参与训练。每天的训练包括网格鼓训练、平衡木训练和棒滚动训练，总时长 40 分钟，每天一次。对照组大鼠不接受训练。在功能训练 14 天后，接受双侧肢体训练的大鼠的神经功能缺损程度比接受同侧肢体训练的大鼠和对照组大鼠轻。在接受双侧肢体训练的大鼠中，梗死区外周和未受影响半球相应脑区的巢蛋白/胶质纤维酸性蛋白阳性细胞和巢蛋白/微管相关蛋白 2 阳性细胞数量明显高于接受同侧肢体训练的大鼠。这些数据表明，脑梗死后双侧肢体训练可促进双侧半球内源性神经干细胞的增殖和分化，加速神经功能的恢复。此外，双侧肢体训练比同侧肢体训练产生更好的治疗效果。
