短期和长期雌二醇或孕酮治疗可改变前额叶皮层和海马中 GFAP、MAP2 和 Tau 的表达。
Short- and long-term treatment with estradiol or progesterone modifies the expression of GFAP, MAP2 and Tau in prefrontal cortex and hippocampus.
机构信息
Departamento de Biología, Universidad Nacional Autónoma de México, Ciudad Universitaria, Coyoacán, Mexico.
出版信息
Life Sci. 2011 Jul 18;89(3-4):123-8. doi: 10.1016/j.lfs.2011.05.008. Epub 2011 Jun 12.
AIMS
We analyzed the effects of the short- and long-term administration of estradiol (E2) or progesterone (P4) after ovariectomy on the expression of MAP2, Tau and GFAP in prefrontal cortex and hippocampus.
MAIN METHODS
Sprague Dawley rats were ovariectomized and immediately treated with E2 or P4 for 2 or 18 weeks. At the end of treatments, hippocampus and prefrontal cortex were excised, proteins were extracted and MAP2, Tau and GFAP were analyzed by Western blot.
KEY FINDINGS
MAP2 and Tau content was not modified by E2 in the prefrontal cortex. On the contrary, P4 decreased MAP2 content after a short-term treatment, while it increased that of MAP2 and TAU in this brain region after a long-term treatment. E2 increased MAP2 content in hippocampus. In this region, short-term administration of P4 increased that of MAP2. GFAP content was diminished after a long-term administration of P4 in hippocampus.
SIGNIFICANCE
Current data emphasize the importance of short- and long-term sex steroid treatment on neuronal and glial cytoskeletal proteins expression.
目的
我们分析了卵巢切除术后短期和长期给予雌二醇（E2）或孕酮（P4）对前额叶皮层和海马中 MAP2、Tau 和 GFAP 表达的影响。
方法
Sprague Dawley 大鼠卵巢切除后立即用 E2 或 P4 处理 2 或 18 周。在治疗结束时，切除海马和前额叶皮层，提取蛋白质，并用 Western blot 分析 MAP2、Tau 和 GFAP。
主要发现
E2 在前额叶皮层中未改变 MAP2 和 Tau 的含量。相反，P4 在短期治疗后降低了 MAP2 的含量，而在长期治疗后增加了 MAP2 和 Tau 在该脑区的含量。E2 增加了海马中 MAP2 的含量。在该区域，P4 的短期给药增加了 MAP2 的含量。海马中 P4 的长期给药会降低 GFAP 的含量。
意义
目前的数据强调了短期和长期性激素治疗对神经元和神经胶质细胞骨架蛋白表达的重要性。
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