Liu Yixiong, Fan Linni, Wang Yingmei, Li Peifeng, Zhu Jin, Wang Lu, Zhang Weichen, Zhang Yuehua, Huang Gaosheng
Department of Pathology, Xijing Hospital, The Fourth Military Medical University Xi'an, China.
Int J Clin Exp Pathol. 2014 Aug 15;7(9):5429-35. eCollection 2014.
To explore the relationship between the number of tumor-associated macrophages (TAMs) and proliferative activity of tumor cells and the relationship between two macrophage biomarkers CD68 and CD163 in nasopharyngeal NK/T-cell lymphoma.
Immunohistochemistry was used to reconfirm the diagnosis of nasal NK/T-cell lymphoma and detect the numbers of TAMs and the ki-67 label index of the tumor cells in all 31 cases. In addition, 12 cases of inflammatory cases were collected as controls, for which the immunostaining of CD68 and CD163 were done as well. Then staining results were analyzed with Pearson correlation and t test.
The number of TAMs was positively correlated with tumor proliferative activity (P = 0.024) in nasopharyngeal NK/T-cell lymphoma. The expression of CD68 and CD163 was closely related (P = 0.009), and the positive rate of CD68 was generally higher than CD163, however there is no statistical significance.
The increase in numbers of TAMs in nasopharyngeal NK/T-cell lymphoma is related to higher proliferative index, indicating the TAMs play an important role in tumor proliferation. Meanwhile both CD68 and CD163 might be the markers for TAMs but CD163 would be the better one.
探讨鼻咽癌NK/T细胞淋巴瘤中肿瘤相关巨噬细胞（TAM）数量与肿瘤细胞增殖活性之间的关系，以及两种巨噬细胞生物标志物CD68和CD163之间的关系。
采用免疫组织化学方法对31例鼻腔NK/T细胞淋巴瘤进行诊断复核，并检测TAM数量及肿瘤细胞的ki-67标记指数。另外，收集12例炎症病例作为对照，同时进行CD68和CD163免疫染色。然后采用Pearson相关性分析和t检验对染色结果进行分析。
鼻咽癌NK/T细胞淋巴瘤中TAM数量与肿瘤增殖活性呈正相关（P = 0.024）。CD68和CD163的表达密切相关（P = 0.009），CD68阳性率总体高于CD163，但差异无统计学意义。
鼻咽癌NK/T细胞淋巴瘤中TAM数量增加与较高的增殖指数相关，表明TAM在肿瘤增殖中起重要作用。同时，CD68和CD163可能均为TAM的标志物，但CD163可能是更好的标志物。
