Huang Cong-Fa, Yu Guang-Tao, Wang Wei-Ming, Liu Bing, Sun Zhi-Jun
The State Key Laboratory Breeding Base of Basic Science of Stomatology & Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University Wuhan, China.
The State Key Laboratory Breeding Base of Basic Science of Stomatology & Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University Wuhan, China ; Department of Oral Maxillofacial-Head Neck Oncology, School and Hospital of Stomatology, Wuhan University Wuhan, China.
Int J Clin Exp Pathol. 2014 Aug 15;7(9):6032-9. eCollection 2014.
SPP1, PAI and caveolin-1 are known to be closely associated with tumor progression in several kinds of human tumors. This study aimed to investigate the expression of SPP1, PAI and caveolin-1 in oral squamous cell carcinoma (OSCC), and to evaluate their association with the prognosis in oral carcinoma. Immunohistochemical staining was used to examine the expression of SPP1, PAI and caveolin-1 in 17 normal oral mucosa, 6 oral epithelial dysplasia and 43 OSCC specimens by tissue microarrays. High expression of SPP1, PAI and caveolin-1 was found in OSCC patients, and SPP1 and PAI expression were significantly higher in OSCC than in normal oral mucosa. No significant correlations were found between SPP1, PAI and caveolin-1 expression and clinicopathological factors. Expression of SPP1, PAI and caveolin-1 was also not associated with overall survival. Moreover, SPP1 was closely correlated with PAI, caveolin-1 and Keap1, and PAI had significant correlations with caveolin-1, Keap1 and Nrf2, and caveolin-1 was associated with Keap1 by using the Pearson correlation coefficient test. Our findings suggest that overexpressed SPP1, PAI and caveolin-1 were linked to carcinogenesis and progression, and thus they may serve as potential prognostic factors in OSCC.
已知分泌性磷蛋白1（SPP1）、纤溶酶原激活物抑制剂（PAI）和小窝蛋白-1（caveolin-1）与多种人类肿瘤的进展密切相关。本研究旨在调查SPP1、PAI和caveolin-1在口腔鳞状细胞癌（OSCC）中的表达情况，并评估它们与口腔癌预后的相关性。采用免疫组织化学染色法，通过组织芯片检测17例正常口腔黏膜、6例口腔上皮发育异常和43例OSCC标本中SPP1、PAI和caveolin-1的表达。在OSCC患者中发现SPP1、PAI和caveolin-1高表达，且OSCC中SPP1和PAI的表达显著高于正常口腔黏膜。未发现SPP1、PAI和caveolin-1的表达与临床病理因素之间存在显著相关性。SPP1、PAI和caveolin-1的表达也与总生存期无关。此外，使用Pearson相关系数检验发现，SPP1与PAI、caveolin-1和 Kelch样环氧氯丙烷相关蛋白1（Keap1）密切相关，PAI与caveolin-1、Keap1和核因子E2相关因子2（Nrf2）显著相关，caveolin-1与Keap1相关。我们的研究结果表明，SPP1、PAI和caveolin-1的过表达与致癌作用和肿瘤进展有关，因此它们可能作为OSCC潜在的预后因素。
