Hu Jian Ming, Sun Qi, Li Ling, Liu Chun Xia, Chen Yun Zhao, Zou Hong, Pang Li Juan, Zhao Jin, Yang Lan, Cao Yu Wen, Cui Xiao Bin, Qi Yan, Liang Wei Hua, Zhang Wen Jie, Li Feng
Department of Pathology and Key Laboratory of Xinjiang Endemic and Ethnic Diseases (Ministry of Education), Shihezi University School of Medicine Shihezi 832002, Xinjiang, China ; Department of Oncology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology Wuhan 430030, China.
Department of Pathology and Key Laboratory of Xinjiang Endemic and Ethnic Diseases (Ministry of Education), Shihezi University School of Medicine Shihezi 832002, Xinjiang, China.
Int J Clin Exp Pathol. 2014 Aug 15;7(9):6165-71. eCollection 2014.
Human papillomavirus (HPV) infection is a major risk factor for cervical cancer. However, only some high risk human papillomavirus (HR-HPV)-infected women progress to cervical cancer, host immunogenetic factors human leukocyte antigen (HLA) may account for viral antigens presenting individually or together in the progression to cervical cancer. This study examined the association between the development of invasive cervical cancer (ICC) and the determinant factors including HLA-DRB11501 and DQB10602, HR-HPV infection among Chinese Uighur and Han populations. Blood samples, cervical swabs and biopsies were obtained from 287 patients with ICC (192 Uighurs and 95 Hans) and 312 healthy controls (218 Uighurs and 94 Hans). HPV DNA was detected by PCR and HLA-DRB11501 and DQB10602 alleles were performed using PCR-SSP method. HPV16 infection rates was significantly higher among Uighur and Han with ICC as compared to healthy controls (OR = 58.317; 95% CI: 39.663-85.744; OR = 33.778; 95% CI: 12.581-90.691; P < 0.05 for all). HLA-DRB11501 (OR = 0.305; 95% CI: 0.115-0.813; P < 0.05) and HLA-DRB11501-DQB10602 haplotype frequencies (OR = 0.274; 95% CI: 0.086-0.874; P < 0.05) were significantly reduced in Han ICC. The HLA-DQB10602 frequency significantly decreased among Uighur women with ICC (OR = 0.482; 95% CI: 0.325-0.716; P < 0.05). Similar tendencies were observed for DQB10602 with HPV16-positive ICC (OR = 0.550; 95% CI: 0.362-0.837; P < 0.05). This study suggests that HLA-DRB11501 and DQB1*0602 alleles may influence the immune response to HPV16 infection and decrease the risk of ICC among Uighurs and Hans in Xinjiang, China.
人乳头瘤病毒（HPV）感染是宫颈癌的主要危险因素。然而，只有部分高危型人乳头瘤病毒（HR-HPV）感染的女性会发展为宫颈癌，宿主免疫遗传因素人类白细胞抗原（HLA）可能在宫颈癌进展过程中单独或共同影响病毒抗原的呈递。本研究调查了中国维吾尔族和汉族人群中浸润性宫颈癌（ICC）的发生与包括HLA-DRB11501和DQB10602在内的决定因素以及HR-HPV感染之间的关联。采集了287例ICC患者（192例维吾尔族和95例汉族）和312例健康对照者（218例维吾尔族和94例汉族）的血液样本、宫颈拭子和活检组织。采用PCR法检测HPV DNA，并用PCR-SSP法检测HLA-DRB11501和DQB10602等位基因。与健康对照相比，维吾尔族和汉族ICC患者的HPV16感染率显著更高（OR = 58.317；95%CI：39.663 - 85.744；OR = 33.778；95%CI：12.581 - 90.691；P均<0.05）。汉族ICC患者中HLA-DRB11501（OR = 0.305；95%CI：0.115 - 0.813；P < 0.05）和HLA-DRB11501-DQB10602单倍型频率（OR = 0.274；95%CI：0.086 - 0.874；P < 0.05）显著降低。维吾尔族ICC女性中HLA-DQB10602频率显著降低（OR = 0.482；95%CI：0.325 - 0.716；P < 0.05）。HPV16阳性的ICC患者中DQB10602也有类似趋势（OR = 0.550；95%CI：0.362 - 0.837；P < 0.05）。本研究表明，HLA-DRB11501和DQB1*0602等位基因可能影响对HPV16感染的免疫反应，并降低中国新疆维吾尔族和汉族人群患ICC的风险。
