Department of Gynecology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, China.
Department of Reproductive Medicine, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, China.
Mediators Inflamm. 2019 Jun 18;2019:6519427. doi: 10.1155/2019/6519427. eCollection 2019.
This study is to investigate the role of regulatory B (Breg) cells in cervical cancer. In total, 70 cases of cervical cancer, 52 cases of cervical intraepithelial neoplasia (CIN), and 40 normal controls were enrolled. The percentage of Breg cells was detected by flow cytometry. Serum levels of IL-10 were measured by ELISA. The correlation between Breg cells and the clinical characterizations of cervical cancer was analyzed. The inhibition effect of Breg cells on CD8 T cells was tested by blocking IL-10 . The percentage of CD19CD5CD1d Breg cells and the level of IL-10 of patients with cervical cancer or CIN were significantly higher than those in the control group ( < 0.05). And the postoperative levels of Breg cells and IL-10 were significantly lower than the preoperative levels ( < 0.05). Breg cells and the IL-10 level were positively correlated in cervical cancer patients ( = 0.516). In addition, the Breg cell percentage was closely related to the FIGO stages, lymph node metastasis, tumor differentiation, HPV infection, and the tumor metastasis of cervical cancer ( < 0.05). The Breg cell percentage was negatively correlated with CD8 T cells of cervical cancer patients ( = -0.669). The level of IL-10 in the culture supernatant of Bregs treated with CpG was significantly higher than that of non-Bregs ( < 0.05). After coculture with Bregs, the quantity of CD8 T cells to secrete perforin and Granzyme B was significantly decreased, and this effect was reversed after blocking IL-10 by a specific antibody. Breg cells are elevated in cervical cancer and associated with disease progression and metastasis. Moreover, they can inhibit the cytotoxicity of CD8 T cells.
本研究旨在探讨调节性 B（Breg）细胞在宫颈癌中的作用。共纳入 70 例宫颈癌患者、52 例宫颈上皮内瘤变（CIN）患者和 40 例正常对照者。采用流式细胞术检测 Breg 细胞比例，ELISA 法检测血清白细胞介素-10（IL-10）水平。分析 Breg 细胞与宫颈癌临床特征的相关性，并用 IL-10 阻断剂检测 Breg 细胞对 CD8 T 细胞的抑制作用。宫颈癌或 CIN 患者的 CD19CD5CD1d Breg 细胞比例和 IL-10 水平显著高于对照组（ < 0.05），且术后 Breg 细胞和 IL-10 水平显著低于术前（ < 0.05）。宫颈癌患者 Breg 细胞比例与 IL-10 水平呈正相关（ = 0.516）。此外，Breg 细胞比例与宫颈癌患者的 FIGO 分期、淋巴结转移、肿瘤分化、HPV 感染及肿瘤转移密切相关（ < 0.05）。Breg 细胞比例与宫颈癌患者 CD8 T 细胞呈负相关（ = -0.669）。CpG 作用后的 Breg 细胞培养上清液中 IL-10 水平明显高于非-Breg 细胞（ < 0.05）。与 Breg 细胞共培养后，CD8 T 细胞分泌穿孔素和 Granzyme B 的数量明显减少，用特异性抗体阻断 IL-10 后，这种作用被逆转。Breg 细胞在宫颈癌中升高，并与疾病进展和转移相关，而且可抑制 CD8 T 细胞的细胞毒性。
