Joyce Andrea L, White William H, Nuessly Gregg S, Solis M Alma, Scheffer Sonja J, Lewis Matthew L, Medina Raul F
SNRI, University of California Merced, Merced, California, United States of America.
USDA-ARS Sugarcane Research Unit, Houma, Louisiana, United States of America.
PLoS One. 2014 Oct 22;9(10):e110036. doi: 10.1371/journal.pone.0110036. eCollection 2014.
The sugarcane borer moth, Diatraea saccharalis, is widespread throughout the Western Hemisphere, and is considered an introduced species in the southern United States. Although this moth has a wide distribution and is a pest of many crop plants including sugarcane, corn, sorghum and rice, it is considered one species. The objective was to investigate whether more than one introduction of D. saccharalis had occurred in the southern United States and whether any cryptic species were present. We field collected D. saccharalis in Texas, Louisiana and Florida in the southern United States. Two molecular markers, AFLPs and mitochondrial COI, were used to examine genetic variation among these regional populations and to compare the sequences with those available in GenBank and BOLD. We found geographic population structure in the southern United States which suggests two introductions and the presence of a previously unknown cryptic species. Management of D. saccharalis would likely benefit from further investigation of population genetics throughout the range of this species.
甘蔗螟蛾（Diatraea saccharalis）广泛分布于西半球，在美国南部被视为外来物种。尽管这种蛾子分布广泛，是甘蔗、玉米、高粱和水稻等多种农作物的害虫，但它被认为是一个物种。目的是调查在美国南部甘蔗螟蛾是否不止一次被引入，以及是否存在任何隐性物种。我们在美国南部的得克萨斯州、路易斯安那州和佛罗里达州实地采集了甘蔗螟蛾。使用两种分子标记，即扩增片段长度多态性（AFLPs）和线粒体细胞色素氧化酶亚基I（COI），来检测这些区域种群之间的遗传变异，并将序列与GenBank和BOLD中可用的序列进行比较。我们发现美国南部存在地理种群结构，这表明有两次引入事件，并且存在一个先前未知的隐性物种。对甘蔗螟蛾的管理可能会受益于对该物种整个分布范围内种群遗传学的进一步研究。
