Alharbi Khalid Khalaf, Khan Imran Ali, Al-Sheikh Yazeed A, Alharbi Fawiziah Khalaf, Alharbi Fahad Khalaf, Al-Nbaheen May Salem
Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh, Kingdom of Saudi Arabia.
Department of Biology Science, College of Science and Arts, Al-Qassim University, Buraidah, Kingdom of Saudi Arabia.
Acta Biochim Pol. 2014;61(4):769-72. Epub 2014 Oct 22.
The ubiquitin-conjugating enzyme E2E 2 (UBE2E2) gene plays an important role in insulin synthe-sis and secretion under conditions in which stress to the endoplasmic reticu-lum is increased in β-cells. In this case-control study, we have selected rs7612462 polymorphism within UBE2E2 gene to identify in a Saudi population the type 2 diabetes mellitus (T2DM) subjects. In total, 376 subjects with T2DM and 380 controls were enrolled in this study. We have collected 5 mL of peripheral blood from each participant for biochemical and molecular analyses. PCR-RFLP was used to generate genotypes at rs7612462 in all of the study subjects. Clinical data and anthropometric measurements of the patients were significantly different from those of the controls (p<0.05). All of the subjects used in this study were non-obese (25<BMI<30). None of the alleles or genotypes of rs7612462 were associated with T2DM (OR=1.251, 95% CI=0.7703-2.034; p=0.3641). Our data suggest that rs7612462 polymorphism in UBE2E2 does not contribute to T2DM susceptibility in the Saudi population.
泛素结合酶E2E 2（UBE2E2）基因在β细胞内质网应激增加的情况下，对胰岛素的合成和分泌起着重要作用。在这项病例对照研究中，我们选择了UBE2E2基因内的rs7612462多态性，以在沙特人群中识别2型糖尿病（T2DM）患者。本研究共纳入376例T2DM患者和380例对照。我们从每位参与者采集了5毫升外周血用于生化和分子分析。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法对所有研究对象的rs7612462位点进行基因分型。患者的临床数据和人体测量指标与对照组有显著差异（p<0.05）。本研究中所有受试者均非肥胖（25<BMI<30）。rs7612462的所有等位基因或基因型均与T2DM无关（OR=1.251，95%CI=0.7703-2.034；p=0.3641）。我们的数据表明，UBE2E2基因中的rs7612462多态性对沙特人群的T2DM易感性没有影响。
