Mergani Adil, Mansour Ahmed Abdelkhalik, Askar Tamer, Zahran Rasha Nabeel, Mustafa Adil Musa, Mohammed Mukhtar Ahmed, Saleh Osama Mosailhy
College of Applied Medical Sciences - Turabah, Taif University, Taif, Saudi Arabia.
Department of Genetics, Faculty of Agriculture, Ain Sham University, Cairo, Egypt.
Biochem Genet. 2016 Aug;54(4):544-551. doi: 10.1007/s10528-016-9740-2. Epub 2016 Jul 1.
Type 2 diabetes mellitus is characterized by chronic hyperglycemia and associated with oxidative stress resulting from accumulation of free radicals in body's tissues, which especially affects beta cells in pancreas and is an important factor in the development of diabetes and its complications. Glutathione S-transferases (GSTs) are a family of antioxidant enzymes that play important roles in decreasing ROS species and act as a kind of antioxidant defense. In a case-control study, we investigated the role of GSTP1 Ile105Val polymorphism in predisposition to T2DM in patients from Tarabah province, Saudi Arabia. The polymorphism was screened by PCR-RFLP in 90 T2DM patients and 87 healthy controls. The genotypes and alleles frequencies in cases and controls were assessed using Cochran-Armitage trend test and odds ratios (ORs), and 95 % confidence intervals (CIs) in different genetic models of inheritance were calculated. Our data indicate that G allele (Val) is associated with an increased risk for T2DM in this population in any combination (OR 4.101, 95 % CI 1.986-8.469, P = 0.00008). This indicates that individuals who are carriers for the mutant allele, either in homozygous (GG) or heterozygous (AG) state, are at fourfold higher risk for development of T2DM than other subjects in this population.
2型糖尿病的特征是慢性高血糖，并与体内组织中自由基积累所导致的氧化应激相关，氧化应激尤其会影响胰腺中的β细胞，是糖尿病及其并发症发生发展的一个重要因素。谷胱甘肽S-转移酶（GSTs）是一类抗氧化酶，在减少活性氧物质方面发挥重要作用，起到一种抗氧化防御的作用。在一项病例对照研究中，我们调查了沙特阿拉伯塔拉巴省患者中GSTP1 Ile105Val多态性在2型糖尿病易感性中的作用。通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）对90例2型糖尿病患者和87例健康对照进行该多态性筛查。使用 Cochr an-Armitage趋势检验和比值比（ORs）评估病例组和对照组的基因型及等位基因频率，并计算不同遗传模式下的95%置信区间（CIs）。我们的数据表明，在该人群中，任何组合下G等位基因（Val）都与2型糖尿病风险增加相关（OR 4.101，95% CI 1.986 - 8.469，P = 0.00008）。这表明，无论是纯合子（GG）还是杂合子（AG）状态下的突变等位基因携带者，患2型糖尿病的风险比该人群中的其他受试者高四倍。
