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通过宏基因组测序对水力压裂源水和天然气开采产出水中微生物群落的功能潜力进行表征。

The functional potential of microbial communities in hydraulic fracturing source water and produced water from natural gas extraction characterized by metagenomic sequencing.

作者信息

Mohan Arvind Murali, Bibby Kyle J, Lipus Daniel, Hammack Richard W, Gregory Kelvin B

机构信息

National Energy Technology Laboratory, Pittsburgh, Pennsylvania, United States of America; Department of Civil and Environmental Engineering, Carnegie Mellon University, Pittsburgh, Pennsylvania, United States of America.

National Energy Technology Laboratory, Pittsburgh, Pennsylvania, United States of America; Department of Civil and Environmental Engineering, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America; Department of Computational and Systems Biology, University of Pittsburgh Medical School, Pittsburgh, Pennsylvania, United States of America.

出版信息

PLoS One. 2014 Oct 22;9(10):e107682. doi: 10.1371/journal.pone.0107682. eCollection 2014.

DOI:10.1371/journal.pone.0107682
PMID:25338024
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4206270/
Abstract

Microbial activity in produced water from hydraulic fracturing operations can lead to undesired environmental impacts and increase gas production costs. However, the metabolic profile of these microbial communities is not well understood. Here, for the first time, we present results from a shotgun metagenome of microbial communities in both hydraulic fracturing source water and wastewater produced by hydraulic fracturing. Taxonomic analyses showed an increase in anaerobic/facultative anaerobic classes related to Clostridia, Gammaproteobacteria, Bacteroidia and Epsilonproteobacteria in produced water as compared to predominantly aerobic Alphaproteobacteria in the fracturing source water. The metabolic profile revealed a relative increase in genes responsible for carbohydrate metabolism, respiration, sporulation and dormancy, iron acquisition and metabolism, stress response and sulfur metabolism in the produced water samples. These results suggest that microbial communities in produced water have an increased genetic ability to handle stress, which has significant implications for produced water management, such as disinfection.

摘要

水力压裂作业产生的采出水中的微生物活动会导致不良的环境影响，并增加天然气生产成本。然而，这些微生物群落的代谢特征尚未得到充分了解。在此，我们首次展示了水力压裂源水和水力压裂产生的废水中微生物群落的鸟枪法宏基因组结果。分类学分析表明，与压裂源水中主要为需氧的变形菌门相比，采出水中与梭菌纲、γ-变形菌纲、拟杆菌纲和ε-变形菌纲相关的厌氧/兼性厌氧类群有所增加。代谢特征显示，采出水样中负责碳水化合物代谢、呼吸作用、孢子形成和休眠、铁获取和代谢、应激反应以及硫代谢的基因相对增加。这些结果表明，采出水中的微生物群落应对压力的遗传能力增强，这对采出水管理（如消毒）具有重要意义。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a933/4206270/80f6926f482e/pone.0107682.g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a933/4206270/ea24e5c6e237/pone.0107682.g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a933/4206270/59f901bcc59c/pone.0107682.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a933/4206270/7925e392c9e6/pone.0107682.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a933/4206270/8b2391e07c0d/pone.0107682.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a933/4206270/9bb4a0eb8730/pone.0107682.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a933/4206270/7d8704e831fa/pone.0107682.g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a933/4206270/80f6926f482e/pone.0107682.g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a933/4206270/ea24e5c6e237/pone.0107682.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a933/4206270/365b1c0ea080/pone.0107682.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a933/4206270/f4966fc7528a/pone.0107682.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a933/4206270/7f659b20e1a5/pone.0107682.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a933/4206270/59f901bcc59c/pone.0107682.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a933/4206270/7925e392c9e6/pone.0107682.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a933/4206270/8b2391e07c0d/pone.0107682.g007.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a933/4206270/80f6926f482e/pone.0107682.g010.jpg

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