Rossmassler Karen, Hanson Thomas E, Campbell Barbara J
School of Marine Science and Policy, College of Earth, Ocean, and Environment, University of Delaware, Lewes, DE, USA.
School of Marine Science and Policy, College of Earth, Ocean, and Environment, University of Delaware, Lewes, DE, USA Department of Biological Sciences, University of Delaware, Newark, DE, USA Delaware Biotechnology Institute, University of Delaware, Newark, DE, USA.
FEMS Microbiol Lett. 2016 Aug;363(16). doi: 10.1093/femsle/fnw162. Epub 2016 Jun 19.
In sulfidic environments, microbes oxidize reduced sulfur compounds via several pathways. We used metagenomics to investigate sulfur metabolic pathways from microbial mat communities in two subterranean sulfidic streams in Lower Kane Cave, WY, USA and from Glenwood Hot Springs, CO, USA. Both unassembled and targeted recA gene assembly analyses revealed that these streams were dominated by Epsilonproteobacteria and Gammaproteobacteria, including groups related to Sulfurovum, Sulfurospirillum, Thiothrix and an epsilonproteobacterial group with no close cultured relatives. Genes encoding sulfide:quinone oxidoreductase (SQR) were abundant at all sites, but the specific SQR type and the taxonomic affiliation of each type differed between sites. The abundance of thiosulfate oxidation pathway genes (Sox) was not consistent between sites, although overall they were less abundant than SQR genes. Furthermore, the Sox pathway appeared to be incomplete in all samples. This work reveals both variations in sulfur metabolism within and between taxonomic groups found in these systems, and the presence of novel epsilonproteobacterial groups.
在硫化环境中，微生物通过多种途径氧化还原态硫化合物。我们利用宏基因组学研究了美国怀俄明州下凯恩洞穴的两条地下硫化溪流以及美国科罗拉多州格伦伍德温泉的微生物垫群落中的硫代谢途径。未组装和靶向recA基因组装分析均显示，这些溪流中以ε-变形菌纲和γ-变形菌纲为主，包括与硫卵菌属、硫螺旋菌属、丝硫菌属相关的类群，以及一个与未培养近缘种关系密切的ε-变形菌类群。编码硫化物：醌氧化还原酶（SQR）的基因在所有位点都很丰富，但各位点的特定SQR类型及其分类归属有所不同。硫代硫酸盐氧化途径基因（Sox）的丰度在位点间不一致，尽管总体上它们比SQR基因的丰度低。此外，所有样品中的Sox途径似乎都是不完整的。这项工作揭示了这些系统中分类群内部和之间硫代谢的差异，以及新型ε-变形菌类群的存在。
