Zhao Jing-lei, Jiang Ling-yong, Mao Li-xia, Liu Jia-qiang, Fang Bing
Department of Oral and Cranomaxillofacial Science, Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; Shanghai Key Laboratory of Stomatology. Shanghai 200011, China.E-mail:
Shanghai Kou Qiang Yi Xue. 2014 Aug;23(4):391-6.
To isolate and identify the human periodontal ligament stem cells, evaluate osteogenetic capacity, and investigate the changes of osteogenic bone related gene expression in mineralized medium at different times.
PDLSCs were isolated by tissue culture and magnetic activated cell sorting. Immunofluorescence staining was used for identification. The general situation of osteogenesis was assessed with alkaline phosphatase (ALP) and alizarin red staining. Real-time PCR was used to detect the expression of genes in osteoinduction. SPSS12.0 software package was used for data analysis.
Tissue culture with magnetic cell sorting could isolate high-purity human periodontal ligament stem cells. During the osteogenic process, the expression of FoxO1 and Runx2 firstly increased and then decreased, ALP and OCN gene levels continued to increase.
Similar to osteogenesis, ALP, Runx2, FoxO1 and OCN are regularly expressed during osteogenic induction.
分离并鉴定人牙周膜干细胞，评估其成骨能力，并研究在矿化培养基中不同时间点成骨相关基因表达的变化。
通过组织培养和磁激活细胞分选分离牙周膜干细胞。采用免疫荧光染色进行鉴定。用碱性磷酸酶（ALP）和茜素红染色评估成骨的总体情况。用实时聚合酶链反应检测成骨诱导过程中基因的表达。使用SPSS12.0软件包进行数据分析。
组织培养结合磁细胞分选可分离出高纯度的人牙周膜干细胞。在成骨过程中，FoxO1和Runx2的表达先升高后降低，ALP和OCN基因水平持续升高。
与成骨过程相似，在成骨诱导过程中，ALP、Runx2、FoxO1和OCN呈规律性表达。
