Southwest Medical University, Lu Zhou, 646000, China.
West China-Guang'an Hospital, Sichuan University, Guang'an, 638550, China.
Lasers Med Sci. 2021 Sep;36(7):1535-1543. doi: 10.1007/s10103-021-03278-1. Epub 2021 Mar 15.
This study aimed to evaluate the effects of low-energy red light-emitting diode (LED) irradiation on the proliferation and osteogenic differentiation of periodontal ligament stem cells (PDLSCs). PDLSCs were derived from human periodontal ligament tissues of premolars and were irradiated with 0 (control group), 1, 3, or 5 J/cm red LED in osteogenic induction medium. Cell proliferation was analyzed using the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay. Osteogenic differentiation activity was evaluated by monitoring alkaline phosphatase (ALP) activity, alizarin red staining, and real-time polymerase chain reaction (RT-PCR) results. Osteoblast-associated proteins (Runx2, OCN, OPN, and BSP) were detected using western blotting. The results of the MTT assay indicated that PDLSCs in the irradiation groups exhibited a higher proliferation rate than those in the control group (P < 0.05). ALP results showed that after 7 days of illumination, only 5 J/cm promoted the expression of ALP of PDLSCs. However, after 14 days of illumination, the irradiation treatments did not increase ALP activity. The results of alizarin red staining showed that red LED promoted osteogenic differentiation of the PDLSCs. The real-time polymerase chain reaction (RT-PCR) results demonstrated that red LED upregulated the expression levels of osteogenic genes. Expression of the proteins BSP, OPN, OCN, and Runx2 in the irradiation groups was higher than that in the control group. Our results confirmed that low-energy red LED at 1, 3, and 5 J/cm promotes proliferation and osteogenic differentiation of PDLSCs.
本研究旨在评估低能量红光发光二极管（LED）照射对牙周膜干细胞（PDLSCs）增殖和成骨分化的影响。PDLSCs 来源于前磨牙的人牙周膜组织，并在成骨诱导培养基中用 0（对照组）、1、3 或 5 J/cm 红光 LED 照射。通过 3-[4,5-二甲基噻唑-2-基]-2,5 二苯基四氮唑溴化物（MTT）测定分析细胞增殖。通过监测碱性磷酸酶（ALP）活性、茜素红染色和实时聚合酶链反应（RT-PCR）结果来评估成骨分化活性。使用蛋白质印迹法检测成骨相关蛋白（Runx2、OCN、OPN 和 BSP）。MTT 测定结果表明，照射组的 PDLSCs 增殖率高于对照组（P<0.05）。ALP 结果表明，仅在照射后 7 天，5 J/cm 促进了 PDLSCs 的 ALP 表达。然而，在照射 14 天后，照射处理并没有增加 ALP 活性。茜素红染色结果表明，红光 LED 促进了 PDLSCs 的成骨分化。实时聚合酶链反应（RT-PCR）结果表明，红光 LED 上调了成骨基因的表达水平。照射组的 BSP、OPN、OCN 和 Runx2 蛋白表达均高于对照组。我们的结果证实，1、3 和 5 J/cm 的低能量红光 LED 促进了 PDLSCs 的增殖和成骨分化。
