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[利用噬菌体M13的DNA分析人类DNA个体间多态性:克拉斯诺达尔市的一项群体研究证明]

[The use of DNA from phage M13 for the analysis of interindividual polymorphism of human DNA as demonstrated by a population study in Krasnodar city].

作者信息

Barysheva E V, Prosniak M I, Vlasov M S, Golubtsov V I, Revazov A A, Limborskaia S A, Ginter E K

出版信息

Genetika. 1989 Nov;25(11):2079-82.

PMID:2533908
Abstract

Hypervariable "minisatellite" regions detected in human genome by wild-type M13 DNA were found to have high polymorphism and somatic stability. Analysis of individual specific patterns of 34 human DNAs from Krasnodar population is presented. The observed length of fragments ranged from 2 to 6 kb. The mean frequency of a fragment in the population under study is p = 0.247 +/- 0.171, the mean number of fragments per individual being x = 9.35 +/- 1.95. The mean probability of individual identification is calculated to be 1-2.10(-6) = 0.999998.

摘要

通过野生型M13 DNA在人类基因组中检测到的高变“微卫星”区域具有高度多态性和体细胞稳定性。本文展示了对克拉斯诺达尔人群34份人类DNA的个体特异性模式分析。观察到的片段长度范围为2至6 kb。在研究人群中,片段的平均频率为p = 0.247 +/- 0.171,每个个体的片段平均数量为x = 9.35 +/- 1.95。计算得出个体识别的平均概率为1 - 2×10⁻⁶ = 0.999998。

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