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[不同分类群生物的基因组指纹图谱:以噬菌体M13 DNA作为杂交探针的应用]

[Genomic fingerprints of organisms from different taxonomic groups: the use of phage M13 DNA as a hybridization probe].

作者信息

Ryskov A P, Dzhincharadze A G, Prosnik M I, Ivanov P L, Limborskaia S A

出版信息

Genetika. 1988 Feb;24(2):227-38.

PMID:3282990
Abstract

Hypervariable polymorphic patterns were detected using wild-type M13 DNA as a probe in genomic DNAs of very different organisms ranging from procaryotes and lower eucaryotes to upper plants and animals, including human beings. Due to somatic stability of highly polymorphic patterns and their discrete inheritance, individual-specific restriction pattern analysis ("DNA fingerprinting") with this test probe was found to be useful in applied human genetics, in particular, for identifying paternity and maternity, and mapping of human genomes. The data obtained also demonstrate some possibilities of the DNA fingerprinting technology in genetics and selection of agricultural plants and animals, such as variety analysis, classification and registration of individual inbred lines and strains, as well as identification of bacterial strains.

摘要

使用野生型M13 DNA作为探针,在从原核生物、低等真核生物到高等植物和动物(包括人类)等非常不同的生物体的基因组DNA中检测到高变多态性模式。由于高多态性模式的体细胞稳定性及其离散遗传,发现使用该测试探针进行个体特异性限制性模式分析(“DNA指纹识别”)在应用人类遗传学中很有用,特别是用于确定亲子关系和人类基因组图谱绘制。获得的数据还证明了DNA指纹识别技术在遗传和农业动植物选择方面的一些可能性,例如品种分析、个体自交系和品系的分类与登记,以及细菌菌株的鉴定。

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