菊花多倍体的微卫星多态性：全基因组复制的影响。
Microsatellite polymorphism among Chrysanthemum sp. polyploids: the influence of whole genome duplication.
作者信息
Wang Haibin, Qi Xiangyu, Gao Ri, Wang Jingjing, Dong Bin, Jiang Jiafu, Chen Sumei, Guan Zhiyong, Fang Weimin, Liao Yuan, Chen Fadi
机构信息
1] College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China [2] Jiangsu Province Engineering Lab for Modern Facility Agriculture Technology &Equipment, Nanjing 210095, China.
College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
出版信息
Sci Rep. 2014 Oct 23;4:6730. doi: 10.1038/srep06730.
Polyploidy is common among flowering plants, including the Asteraceae, a relatively recent angiosperm group. EST-SSRs were used to characterize polymorphism among 29 Chrysanthemum and Ajania spp. accessions of various ploidy levels. Most EST-SSR loci were readily transferable between the species, 29 accessions were separated into three groups in terms of the number of fragments. It inferred that the formation from tetraploid to hexaploid and from octoploid to decaploid may be a recent event, while from the diploid to the tetraploid may be an ancient one in the Chrysanthemum lineage. EST-SSR polymorphism was found and some transcripts containing an SSR were transcribed differently in the de novo autotetraploid C. nankingense and C. lavandulifolium than in their progenitor diploid. EST-SSR could provide a potential molecular basis of adaptation during evolution, while whole genome duplication has a major effect on the mutational dynamics of EST-SSR loci, which could also affect gene regulation.
多倍体在开花植物中很常见，包括菊科植物，菊科是一个相对较新的被子植物类群。利用EST-SSR对29个不同倍性水平的菊花属和亚菊属植物种质的多态性进行了表征。大多数EST-SSR位点很容易在物种间转移，29个种质根据片段数量被分为三组。推测从四倍体到六倍体以及从八倍体到十倍体的形成可能是近期发生的事件，而在菊花谱系中从二倍体到四倍体的形成可能是一个古老的事件。在新形成的同源四倍体南京菊和香叶菊中发现了EST-SSR多态性，并且一些含有SSR的转录本在转录上与它们的二倍体祖先有所不同。EST-SSR可以为进化过程中的适应提供潜在的分子基础，而全基因组加倍对EST-SSR位点的突变动态有重大影响，这也可能影响基因调控。
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