Gao Ri, Wang Haibin, Dong Bin, Yang Xiaodong, Chen Sumei, Jiang Jiafu, Zhang Zhaohe, Liu Chen, Zhao Nan, Chen Fadi
College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
Department of Horticulture, Agricultural College Yanbian University, Park Road 977, Yanji 133002, China.
Int J Mol Sci. 2016 Oct 9;17(10):1690. doi: 10.3390/ijms17101690.
Autopolyploidy is widespread in higher plants and plays an important role in the process of evolution. The present study successfully induced autotetraploidys from by colchicine. The plant morphology, genomic, transcriptomic, and epigenetic changes between tetraploid and diploid plants were investigated. Ligulate flower, tubular flower and leaves of tetraploid plants were greater than those of the diploid plants. Compared with diploid plants, the genome changed as a consequence of polyploidization in tetraploid plants, namely, 1.1% lost fragments and 1.6% novel fragments occurred. In addition, DNA methylation increased after genome doubling in tetraploid plants. Among 485 common transcript-derived fragments (TDFs), which existed in tetraploid and diploid progenitors, 62 fragments were detected as differentially expressed TDFs, 6.8% of TDFs exhibited up-regulated gene expression in the tetraploid plants and 6.0% exhibited down-regulation. The present study provides a reference for further studying the autopolyploidization role in the evolution of In conclusion, the autopolyploid showed a global change in morphology, genome and gene expression compared with corresponding diploid.
同源多倍体在高等植物中广泛存在，并且在进化过程中发挥着重要作用。本研究成功地通过秋水仙素诱导[植物名称]产生了同源四倍体。对四倍体和二倍体植物之间的植物形态、基因组、转录组和表观遗传变化进行了研究。四倍体植物的舌状花、管状花和叶片均大于二倍体植物。与二倍体植物相比，四倍体植物因多倍体化导致基因组发生变化，即1.1%的片段丢失，1.6%的新片段出现。此外，四倍体植物基因组加倍后DNA甲基化增加。在四倍体和二倍体亲本中存在的485个常见转录本衍生片段（TDFs）中，有62个片段被检测为差异表达TDFs，其中6.8%的TDFs在四倍体植物中基因表达上调，6.0%表现为下调。本研究为进一步研究同源多倍体化在[植物名称]进化中的作用提供了参考。总之，与相应的二倍体相比，同源四倍体在形态、基因组和基因表达方面呈现出全局性变化。
