Boancă Mihaela, Popa Eliza Graţiela, Lupuşoru R V, Poroch V, Mititelu-Tarţău Liliana, Lupuşoru Cătălina Elena
Rev Med Chir Soc Med Nat Iasi. 2014 Jul-Sep;118(3):847-53.
The study investigates the effects of magnesium nanovesicles on the memory processes performance in mice.
L-a-phosphatidylcholine was used to obtain nano formulations as lipid vesicles systems stabilized thereafter with chitosan. The experiment was carried out on white Swiss mice, divided into 3 groups of 7 animals each, treated orally 7 consecutive days: Group I (Control): 0.1 mL/10g distilled water; Group II (Mg): 1 mmol/kbw magnesium chloride; Group III (Mg-vesicles): 1 mmol/kbw magnesium nanovesicles. The spatial memory performance was assessed by recording spontaneous alternation behavior in Y-maze test. Each animal was placed at the end of one arm and allowed to move freely through the maze during a single 8 min session. Alternation was defined as a consecutive entry in three different arms. The alternation percentage was computed according to the formula: (number of alternations/total number of arm visits--2) x 100. Data were analyzed using SPSS 17.0 software. Experimental protocols were implemented according to the recommendations of the University Committee for Research and Ethical Issues.
New carrier formulations entrapping magnesium chloride were designed: their mean size was 129.56 nm and the mean Zeta potential was +36.1 mV, indicating a moderate stability of the solution. Oral administration of magnesium vesicles resulted in a significant increase of spontaneous alternation percent in Y-maze test (p < 0.01), which suggests an improvement of short-term memory.
Using magnesium chloride entrapped in lipid vesicles induced an enhancement of cognitive functions in mice especially by facilitation of learning extinction.
本研究调查镁纳米囊泡对小鼠记忆过程表现的影响。
使用L-α-磷脂酰胆碱获得纳米制剂，作为随后用壳聚糖稳定的脂质囊泡系统。实验在瑞士小白鼠身上进行，将其分为3组，每组7只动物，连续7天口服给药：第一组（对照组）：0.1 mL/10g蒸馏水；第二组（镁组）：1 mmol/kbw氯化镁；第三组（镁囊泡组）：1 mmol/kbw镁纳米囊泡。通过记录Y迷宫试验中的自发交替行为来评估空间记忆表现。将每只动物放置在一个臂的末端，并在单次8分钟的实验过程中让其自由穿过迷宫。交替被定义为连续进入三个不同的臂。交替百分比根据公式计算：（交替次数/总臂访问次数 - 2）×100。使用SPSS 17.0软件分析数据。实验方案根据大学研究与伦理问题委员会的建议实施。
设计了包封氯化镁的新型载体制剂：其平均尺寸为129.56 nm，平均ζ电位为+36.1 mV，表明溶液具有适度的稳定性。口服镁囊泡导致Y迷宫试验中自发交替百分比显著增加（p < 0.01），这表明短期记忆得到改善。
使用包封在脂质囊泡中的氯化镁可增强小鼠的认知功能，特别是通过促进学习消退。
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