Liliana Mititelu-Tartau, Lacramioara Ochiuz, Catalina Elena Lupusoru, Andra Sabina Neculai-Valeanu, Gabriela Rusu, Gratiela Popa
Rev Med Chir Soc Med Nat Iasi. 2015 Jan-Mar;119(1):185-92.
The purpose of our study was the experimental research on the effects of pregabalin in two behavioural models in mice.
The experiment was carried out with white Swiss mice treated intraperitoneally as follows: Group I (Control): distilled water 0.1 ml/10 g body weight; Group II (PGB 10): 10 mg/kbw pregabalin; Group III (PGB 20): 20 mg/kbw pregabalin. The psychomotor abilities of pregabalin were tested on a LE-8811 Actimeter device (Panlab), in order to investigate both global motor behaviour and number of escape attempts during an eight-minute interval session. The exploration of memory processes performance was assessed using the Y-maze model, based on the natural tendency of mice to explore new environment. Data were analyzed using SPSS 13.0 for Windows software. The experimental protocols were implemented according the guidelines of "Grigore T. Popa" University Committee for Research and Ethical Issues.
The administration of pregabalin resulted in a dose-dependent reduction of mice horizontal and vertical movements, statistically significant compared to the Control group. The administration of both PGB10 and PGB20 induced no modifications of the spontaneous alternation percent; also, it did not influence the arm entries number compared to Control group in Y-maze test.
The results reflect a significant dose-dependent diminution of number of escape attempts, exploratory and self-maintenance spontaneous behavior after pregabalin treatment, which could be correlated with an anxiolytic effect. Moreover, the study proved that pregabalin did not modify the animal cognitive processes performance or influence short-term memory of mice in the Y-maze test.
我们研究的目的是在小鼠的两种行为模型中对普瑞巴林的作用进行实验研究。
实验用白色瑞士小鼠，腹腔注射给药，具体如下：第一组（对照组）：蒸馏水0.1 ml/10 g体重；第二组（PGB 10）：普瑞巴林10 mg/kg体重；第三组（PGB 20）：普瑞巴林20 mg/kg体重。在LE - 8811活动计（Panlab）装置上测试普瑞巴林的精神运动能力，以研究八分钟间隔期间的整体运动行为和逃避尝试次数。基于小鼠探索新环境的自然倾向，使用Y迷宫模型评估记忆过程表现。数据使用SPSS 13.0 for Windows软件进行分析。实验方案按照“格里戈尔·T·波帕”大学研究与伦理问题委员会的指导方针实施。
普瑞巴林给药导致小鼠水平和垂直运动呈剂量依赖性减少，与对照组相比有统计学意义。给予PGB10和PGB20均未引起自发交替百分比的改变；此外，在Y迷宫试验中，与对照组相比，它也不影响进入臂的次数。
结果反映出普瑞巴林治疗后逃避尝试次数、探索和自我维持自发行为显著减少且呈剂量依赖性，这可能与抗焦虑作用相关。此外，该研究证明普瑞巴林在Y迷宫试验中未改变动物认知过程表现或影响小鼠的短期记忆。
